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From Silver Plates to Spherical Nanoparticles: Snapshots of Microwave-Assisted Polyol Synthesis.

ABSTRACT: The fabrication of silver nanoparticles (Ag NPs) with different sizes by microwave (MW)-assisted synthesis is presented. The fast heating of the MW technique, combined with the possibility to thermally quench the reactions, enabled us to capture snapshots of nucleation and growth processes difficult to accomplish in other techniques. The Ag NPs were synthesized using poly(vinylpyrrolidone) (PVP) through a polyol approach. The effects of the reaction time, the reaction temperatures, and the silver precursor concentration were investigated. The influence of agitation, the PVP concentration, and the initial conditions of the silver precursor was also studied. It is found that at very short reaction times and at low temperatures, polyhedral plates are formed with sizes ca. 300 nm and large polydispersity. However, by increasing the time or the temperature, a size and shape refinement is observed resulting in 10 nm spherical NPs with low polydispersity. Mechanistic insights are provided based on the observations extracted from transmission electron microscopy (TEM) and ultraviolet-visible spectroscopy (UV-vis). A formation mechanism starting from kinetically favored silver polyhedral plates to thermodynamically favored spherical nanoparticles is proposed. Understanding these effects allowed us to control the particle size and the tuning of Ag NPs on-demand. Moreover, the reproducibility and scalability of the process and the long-term stability of the NPs in aqueous solutions are demonstrated. Finally, we provide a recommendation regarding the use of fresh PVP as a capping and stabilizing agent.

SUBMITTER: Torras M

PROVIDER: S-EPMC7097931 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:These days, research in agriculture is focusing on the theme of sustainability along with protection of agriculture produce. Nanotechnology in the agriculture sector aims for the enhancement of agricultural produce and the reduction of pesticides through providing innovative agrochemical agents and their novel delivery mechanisms. The current investigation involved the green synthesis of silver nanoparticles (AgNPs) from the aqueous leaf extract of Melia azedarach by following a microwave-assisted method to control Fusarium oxysporum, the causal agent of tomato wilt. Biosynthesized Melia leaf extract (MLE)-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDX) spectrometry, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and zeta potential analysis. The intensity of the peak at 434 nm in UV-vis spectra, attributed to the surface plasmon resonance of MLE-AgNPs, changes with reaction parameters. TEM exhibits spherical shaped nanoparticles with an average particle size range from 12 to 46 nm. Efficient inhibition of F. oxysporum, the causal agent of tomato wilt, was achieved after exposure to MLE-AgNPs both in vivo and in vitro. In vitro studies exhibited repressed fungal mycelial growth with 79-98% inhibition as compared to the control. Significant increases in growth parameters of tomato seedlings were observed after treatment with biosynthesized nanoparticles as compared to F. oxysporum-infected plants grown without them under greenhouse conditions. Furthermore, SEM imaging was done to reveal the prominent damage on the cell wall of hyphae and spores after MLE-AgNP treatment. Propidium iodide (PI) staining of mycelium indicated the extent of cell death, causing irretrievable damage and disintegration of cellular membranes by altering the membrane permeability. Also, 2',7'-dichlorofluorescin diacetate (DCFH-DA) fluorescence specifies intracellular reactive oxygen species (ROS) production in F. oxysporum after treatment with MLE-AgNPs. The current investigation suggested that biosynthesized nanoparticles can revolutionize the field of plant pathology by introducing an environment-friendly approach for disease management and playing a potential part in agriculture industry. However, to date, little work has been done to integrate nanotechnology into phytopathology so, this area of research is in need of adoption and exploration for the management of plant diseases.

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From Silver Plates to Spherical Nanoparticles: Snapshots of Microwave-Assisted Polyol Synthesis.

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