Ontology highlight
ABSTRACT:
SUBMITTER: Ciragan A
PROVIDER: S-EPMC7098700 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Ciragan Annika A Backlund Sofia M SM Mikula Kornelia M KM Beyer Hannes M HM Samuli Ollila O H OH Iwaï Hideo H
Frontiers in chemistry 20200319
The growing understanding of partially unfolded proteins increasingly points to their biological relevance in allosteric regulation, complex formation, and protein design. However, the structural characterization of disordered proteins remains challenging. NMR methods can access both the dynamics and structures of such proteins, yet suffering from a high degeneracy of NMR signals. Here, we overcame this bottleneck utilizing a salt-inducible split intein to produce segmentally isotope-labeled sam ...[more]