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The effect of heavy metals on the nutritional value of Alfalfa: comparison of nutrients and heavy metals of Alfalfa (Medicago sativa) in industrial and non-industrial areas.

ABSTRACT: The aim of this study is to compare the nutritional value of Alfalfa and accumulation of heavy metals in the farms near and far from the industrial regions. Three regions were considered located at 2, 32 and 65 km distances from an industrial region, and the nutrient content of the Alfalfa including crude protein, crude fiber, crude fat, nitrogen-free extract, and Ash as well as soil and plant heavy metals was determined. The results showed no significant difference in the value of nutrients in the three regions except nitrogen-free extract (mainly starch and sugars). A positive correlation was observed between nitrogen-free extract and lead, chromium, and arsenic (p ≤ 0.05). In addition, the highest accumulations of heavy metals such as arsenic, chromium, lead and cadmium were found in soil and Alfalfa produced at 2 km distance from the industrial area. The lead and cadmium concentrations were higher than the maximum allowable agricultural soil concentration in the areas near industrial region; the accumulation of these metals in the Alfalfa was however lower than the cattle and plant risk levels. The distribution of heavy metals in the Alfalfa cultivated in these three areas (zinc > copper > lead > chromium > arsenic > cadmium) did not coincide with the average of these metals in the soils (lead > zinc > chromium > copper > cadmium > arsenic). The positive correlation was also recorded between electrical conductivity of agricultural soils and copper, lead, chromium and arsenic content of Alfalfa. The highest translocation factors of arsenic, chromium and lead elements were detected in industrial areas. For copper and zinc, the highest translocation factor was found in non- industrial areas. The results of this study can be applied as an important control program in different areas.

SUBMITTER: Rezaeian M

PROVIDER: S-EPMC7099108 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundNucleorhabdoviruses possess bacilliform particles which contain a single-stranded negative-sense RNA genome. They replicate and mature in the nucleus of infected cells. Together with viruses of three other genera of the family Rhabdoviridae, they are known to infect plants and can be transmitted by arthropod vectors, during vegetative propagation, or by mechanical means. In 2010, an alfalfa (Medicago sativa) plant showing virus-like symptoms was collected from Stadl-Paura, Austria and sent to Julius Kühn Institute for analysis.MethodsElectron microscopy (EM) of leaf extracts from infected plants revealed the presence of rhabdovirus-like particles and was further used for ultrastructural analyses of infected plant tissue. Partially-purified preparations of rhabdovirus nucleocapsids were used for raising an antiserum. To determine the virus genome sequence, high throughput sequencing (HTS) was performed. RT-PCR primers were designed to confirm virus infection and to be used as a diagnostic tool.ResultsEM revealed bacilliform virions resembling those of plant-infecting rhabdoviruses. HTS of ribosomal RNA-depleted total RNA extracts revealed a consensus sequence consisting of 13,875 nucleotides (nt) and containing seven open reading frames (ORFs). Homology and phylogenetic analyses suggest that this virus isolate represents a new species of the genus Nucleorhabdovirus (family Rhabdoviridae). Since the virus originated from an alfalfa plant in Austria, the name alfalfa-associated nucleorhabdovirus (AaNV) is proposed. Viroplasms (Vp) and budding virions were observed in the nuclei of infected cells by EM, thus confirming its taxonomic assignment based on sequence data.ConclusionsIn this study, we identified and characterised a new nucleorhabdovirus from alfalfa. It shared only 39.8% nucleotide sequence identity with its closest known relative, black currant-associated rhabdovirus 1. The virus contains an additional open reading frame (accessory gene) with unknown function, located between the matrix protein and the glycoprotein genes. Serological and molecular diagnostic assays were designed for future screening of field samples. Further studies are needed to identify other natural hosts and potential vectors.

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The effect of heavy metals on the nutritional value of Alfalfa: comparison of nutrients and heavy metals of Alfalfa (Medicago sativa) in industrial and non-industrial areas.

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