Ontology highlight
ABSTRACT:
SUBMITTER: Chandrasekar A
PROVIDER: S-EPMC7100760 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Chandrasekar A A Raja A A Dhinakar Raj G G Thangavelu A A Kumanan K K
Proceedings of the National Academy of Sciences, India. Section B 20150304 3
A reverse-transcription loop mediated isothermal amplification (RT-LAMP) was developed for rapid diagnosis of infectious bronchitis (IB) in poultry by targeting the spike protein 2 gene (S2). RT-LAMP primers were designed for IBV-S2 targets and optimized to run at 60 °C for 45 min. As compared with RT-PCR, RT-LAMP was 100 times more sensitive for IBV-S2 gene. RT-LAMP showed specific amplification with IB viral genome but not with other avian respiratory pathogens due to their mismatching with IB ...[more]