Ontology highlight
ABSTRACT:
SUBMITTER: Zwaagstra KA
PROVIDER: S-EPMC265000 | biostudies-other | 1992 Jan
REPOSITORIES: biostudies-other
Zwaagstra K A KA van der Zeijst B A BA Kusters J G JG
Journal of clinical microbiology 19920101 1
A rapid and sensitive method for the detection and unambiguous typing of infectious bronchitis virus (IBV) is described. RNA was isolated from IBV-infected allantoic fluid and was transcribed into cDNA. This cDNA was amplified by the polymerase chain reaction. The polymerase chain reaction products were subsequently analyzed on an agarose gel. The presence of IBV-specific RNA in the allantoic fluid then allowed the amplification of a 438-bp DNA fragment from the nucleocapsid (N) gene. For the ty ...[more]