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Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2).


ABSTRACT: PURPOSE:Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response. METHODS:The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. RESULTS:LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-?, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells. CONCLUSIONS:LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease.

SUBMITTER: Runfeng L 

PROVIDER: S-EPMC7102548 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2).

Runfeng Li L   Yunlong Hou H   Jicheng Huang H   Weiqi Pan P   Qinhai Ma M   Yongxia Shi S   Chufang Li L   Jin Zhao Z   Zhenhua Jia J   Haiming Jiang J   Kui Zheng Z   Shuxiang Huang H   Jun Dai D   Xiaobo Li L   Xiaotao Hou H   Lin Wang W   Nanshan Zhong Z   Zifeng Yang Y  

Pharmacological research 20200320


<h4>Purpose</h4>Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response.<h4>Methods</h4>The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cell  ...[more]

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