Ontology highlight
ABSTRACT:
SUBMITTER: Lau LT
PROVIDER: S-EPMC7111096 | biostudies-literature | 2003 Dec
REPOSITORIES: biostudies-literature
Lau Lok Ting LT Fung Yin-Wan Wendy YW Wong Freda Pui-Fan FP Lin Selma Sau-Wah SS Wang Chen Ran CR Li Hui Li HL Dillon Natalie N Collins Richard A RA Tam John Siu-Lun JS Chan Paul K S PK Wang Chen G CG Yu Albert Cheung-Hoi AC
Biochemical and biophysical research communications 20031201 4
An enhanced polymerase chain reaction (PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) was developed in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. Clinical samples were collected from 120 patients diagnosed as suspected or probable SARS cases and analyzed by conventional PCR followed by agarose gel electrophoresis, conventional TaqMan real-time PCR, and our enhanced TaqMan real-time PCR assays. An amplic ...[more]