Unknown

Dataset Information

0

Characterization of a novel bat-HKU2-like swine enteric alphacoronavirus (SeACoV) infection in cultured cells and development of a SeACoV infectious clone.


ABSTRACT: Swine enteric alphacoronavirus (SeACoV), also known as swine acute diarrhea syndrome coronavirus (SADS-CoV), belongs to the species Rhinolophus bat coronavirus HKU2. Herein, we report on the primary characterization of SeACoV in vitro. Four antibodies against the SeACoV spike, membrane, nucleocapsid and nonstructural protein 3 capable of reacting with viral antigens in SeACoV-infected Vero cells were generated. We established a DNA-launched SeACoV infectious clone based on the cell adapted passage-10 virus and rescued the recombinant virus with a unique genetic marker in cultured cells. Six subgenomic mRNAs containing the leader-body junction sites, including a bicistronic mRNA encoding the accessory NS7a and NS7b genes, were experimentally identified in SeACoV-infected cells. Cellular ultrastructural changes induced by SeACoV infection were visualized by electron microscopy. The availability of the SeACoV infectious clone and a panel of antibodies against different viral proteins will facilitate further studies on understanding the molecular mechanisms of SeACoV replication and pathogenesis.

SUBMITTER: Yang YL 

PROVIDER: S-EPMC7112019 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterization of a novel bat-HKU2-like swine enteric alphacoronavirus (SeACoV) infection in cultured cells and development of a SeACoV infectious clone.

Yang Yong-Le YL   Liang Qi-Zhang QZ   Xu Shu-Ya SY   Mazing Evgeniia E   Xu Guo-Han GH   Peng Lei L   Qin Pan P   Wang Bin B   Huang Yao-Wei YW  

Virology 20190809


Swine enteric alphacoronavirus (SeACoV), also known as swine acute diarrhea syndrome coronavirus (SADS-CoV), belongs to the species Rhinolophus bat coronavirus HKU2. Herein, we report on the primary characterization of SeACoV in vitro. Four antibodies against the SeACoV spike, membrane, nucleocapsid and nonstructural protein 3 capable of reacting with viral antigens in SeACoV-infected Vero cells were generated. We established a DNA-launched SeACoV infectious clone based on the cell adapted passa  ...[more]

Similar Datasets

| S-EPMC5572857 | biostudies-literature
| S-EPMC7117260 | biostudies-literature
| S-EPMC7094983 | biostudies-literature
| S-EPMC7229464 | biostudies-literature
| S-EPMC3317462 | biostudies-literature
| S-EPMC7117411 | biostudies-literature
| S-EPMC2588415 | biostudies-literature
| S-EPMC8953627 | biostudies-literature
| S-EPMC3283524 | biostudies-literature
| S-EPMC3624308 | biostudies-literature