Ontology highlight
ABSTRACT:
SUBMITTER: Madhugiri R
PROVIDER: S-EPMC7112051 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Madhugiri Ramakanth R Karl Nadja N Petersen Daniel D Lamkiewicz Kevin K Fricke Markus M Wend Ulrike U Scheuer Robina R Marz Manja M Ziebuhr John J
Virology 20171206
Structure predictions suggest a partial conservation of RNA structure elements in coronavirus terminal genome regions. Here, we determined the structures of stem-loops (SL) 1 and 2 of two alphacoronaviruses, human coronavirus (HCoV) 229E and NL63, by RNA structure probing and studied the functional relevance of these putative cis-acting elements. HCoV-229E SL1 and SL2 mutants generated by reverse genetics were used to study the effects on viral replication of single-nucleotide substitutions pred ...[more]