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Genotype shift in human coronavirus OC43 and emergence of a novel genotype by natural recombination.


ABSTRACT: BACKGROUND:Human coronavirus (HCoV) OC43 is the most prevalent HCoV in respiratory tract infections. Its molecular epidemiological characterization, particularly the genotyping, was poorly addressed. METHODS:The full-length spike (S), RNA-dependent RNA polymerase (RdRp), and nucleocapsid (N) genes were amplified from each respiratory sample collected from 65 HCoV-OC43-positive patients between 2005 and 2012. Genotypes were determined by phylogenetic analysis. Recombination was analyzed based on full-length viral genome sequences. Clinical manifestations of each HCoV genotype infection were compared by reviewing clinical records. RESULTS:Sixty of these 65 samples belong to genotypes B, C and D. The remaining five strains had incongruent positions in the phylogenetic trees of the S, RdRp and N genes, suggesting a novel genotype emerging, designated as genotype E. Whole genome sequencing and bootscan analysis indicated that genotype E is generated by recombination between genotypes B, C and D. Temporal analysis revealed a sequential genotype replacement of C, B, D and E over the study period with genotype D being the dominant genotype since 2007. The novel genotype E was only detected in children younger than three years suffering from lower respiratory tract infections. CONCLUSIONS:Our results suggest that HCoV-OC43 genotypes are evolving. Such genotype shift may be an adapting mechanism for HCoV-OC43 maintaining its epidemic.

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7112537 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Genotype shift in human coronavirus OC43 and emergence of a novel genotype by natural recombination.

Zhang Yue Y   Li Jianguo J   Xiao Yan Y   Zhang Jing J   Wang Ying Y   Chen Lan L   Paranhos-Baccalà Gláucia G   Ren Lili L   Wang Jianwei J  

The Journal of infection 20141218 6


<h4>Background</h4>Human coronavirus (HCoV) OC43 is the most prevalent HCoV in respiratory tract infections. Its molecular epidemiological characterization, particularly the genotyping, was poorly addressed.<h4>Methods</h4>The full-length spike (S), RNA-dependent RNA polymerase (RdRp), and nucleocapsid (N) genes were amplified from each respiratory sample collected from 65 HCoV-OC43-positive patients between 2005 and 2012. Genotypes were determined by phylogenetic analysis. Recombination was ana  ...[more]

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