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Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus.


ABSTRACT: The availability of monoclonal antibodies (mAbs) specific for the SARS-coronavirus (SARS-CoV) is important for the development of both diagnostic tools and treatment of infection. A molecular characterization of nine monoclonal antibodies raised in immune mice, using highly purified, inactivated SARS-CoV as the inoculating antigen, is presented in this report. These antibodies are specific for numerous viral protein targets, and six of them are able to effectively neutralize SARS-CoV in vitro, including one with a neutralizing titre of 0.075 nM. A phylogenetic analysis of the heavy and light chain sequences reveals that the mAbs share considerable homology. The majority of the heavy chains belong to a single Ig germline V-gene family, while considerably more sequence variation is evident in the light chain sequences. These analyses demonstrate that neutralization ability can be correlated with specific murine V(H)-gene alleles. For instance, one evident trend is high sequence conservation in the V(H) chains of the neutralizing mAbs, particularly in CDR-1 and CDR-2. The results suggest that optimization of murine mAbs for neutralization of SARS-CoV infection will likely be possible, and will aid in the development of diagnostic tools and passive treatments for SARS-CoV infection.

SUBMITTER: Gubbins MJ 

PROVIDER: S-EPMC7112650 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature

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Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus.

Gubbins Michael J MJ   Plummer Frank A FA   Yuan Xin Y XY   Johnstone Darrell D   Drebot Mike M   Andonova Maya M   Andonov Anton A   Berry Jody D JD  

Molecular immunology 20050101 1


The availability of monoclonal antibodies (mAbs) specific for the SARS-coronavirus (SARS-CoV) is important for the development of both diagnostic tools and treatment of infection. A molecular characterization of nine monoclonal antibodies raised in immune mice, using highly purified, inactivated SARS-CoV as the inoculating antigen, is presented in this report. These antibodies are specific for numerous viral protein targets, and six of them are able to effectively neutralize SARS-CoV in vitro, i  ...[more]

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