Ontology highlight
ABSTRACT:
SUBMITTER: Lemmermeyer T
PROVIDER: S-EPMC7117465 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Lemmermeyer Tanja T Lamp Benjamin B Schneider Rainer R Ziebuhr John J Tekes Gergely G Thiel Heinz-Jürgen HJ
Veterinary microbiology 20151231
Feline coronaviruses (FCoVs) encode five accessory proteins termed 3a, 3b, 3c, 7a and 7b of unknown function. These proteins are dispensable for viral replication in vitro but are supposed to play a role in virulence. In the current study, we produced and characterized 7b-specific monoclonal antibodies (mAbs). A recombinant form of the 7b protein was expressed as a fusion protein in Escherichia coli, purified by immobilized metal affinity chromatography and used as immunogen. Two hybridoma lines ...[more]