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Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.

ABSTRACT: The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic ?-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.

SUBMITTER: Carter SD 

PROVIDER: S-EPMC7112762 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.

Carter Stephen D SD   Hampton Cheri M CM   Langlois Robert R   Melero Roberto R   Farino Zachary J ZJ   Calderon Michael J MJ   Li Wen W   Wallace Callen T CT   Tran Ngoc Han NH   Grassucci Robert A RA   Siegmund Stephanie E SE   Pemberton Joshua J   Morgenstern Travis J TJ   Eisenman Leanna L   Aguilar Jenny I JI   Greenberg Nili L NL   Levy Elana S ES   Yi Edward E   Mitchell William G WG   Rice William J WJ   Wigge Christoph C   Pilli Jyotsna J   George Emily W EW   Aslanoglou Despoina D   Courel Maïté M   Freyberg Robin J RJ   Javitch Jonathan A JA   Wills Zachary P ZP   Area-Gomez Estela E   Shiva Sruti S   Bartolini Francesca F   Volchuk Allen A   Murray Sandra A SA   Aridor Meir M   Fish Kenneth N KN   Walter Peter P   Balla Tamas T   Fass Deborah D   Wolf Sharon G SG   Watkins Simon C SC   Carazo José María JM   Jensen Grant J GJ   Frank Joachim J   Freyberg Zachary Z  

Science advances 20200401 14

The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We sho  ...[more]

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