Ontology highlight
ABSTRACT:
SUBMITTER: Chen HT
PROVIDER: S-EPMC7112806 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Chen Hao-tai HT Zhang Jie J Yang Sheng-hai SH Ma Li-na LN Ma Yan-ping YP Liu Xiang-tao XT Cai Xue-peng XP Zhang Yong-guang YG Liu Yong-sheng YS
Journal of virological methods 20090212 1-2
A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine parvovirus (PPV) DNA. The amplification could be finished in 45 min under isothermal condition at 62 degrees C by employing a set of four primers targeting VP2 gene of PPV. LAMP assay showed higher sensitivity than PCR, with a detection limit of 5 copies of PPV genomic DNA per reaction. No cross reactivity was observed from the samples of other related viruses includi ...[more]