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Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification.

ABSTRACT: A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine parvovirus (PPV) DNA. The amplification could be finished in 45 min under isothermal condition at 62 degrees C by employing a set of four primers targeting VP2 gene of PPV. LAMP assay showed higher sensitivity than PCR, with a detection limit of 5 copies of PPV genomic DNA per reaction. No cross reactivity was observed from the samples of other related viruses including canine parvovirus, parvovirus B19, porcine circovirus type 1, porcine circovirus type 2 and porcine peudorabies virus. The detection rate of PPV LAMP for 125 clinical samples was 97.6% and appeared higher than that of PCR method. The result indicated the potential usefulness of the technique as a simple, rapid procedure for the detection of PPV.

SUBMITTER: Chen HT

PROVIDER: S-EPMC7112806 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification.

Chen Hao-tai HT Zhang Jie J Yang Sheng-hai SH Ma Li-na LN Ma Yan-ping YP Liu Xiang-tao XT Cai Xue-peng XP Zhang Yong-guang YG Liu Yong-sheng YS

Journal of virological methods 20090212 1-2

A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine parvovirus (PPV) DNA. The amplification could be finished in 45 min under isothermal condition at 62 degrees C by employing a set of four primers targeting VP2 gene of PPV. LAMP assay showed higher sensitivity than PCR, with a detection limit of 5 copies of PPV genomic DNA per reaction. No cross reactivity was observed from the samples of other related viruses includi ...[more]

PMID: 19428576

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