Ontology highlight
ABSTRACT:
SUBMITTER: Chen HT
PROVIDER: S-EPMC7112855 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Chen Hao-Tai HT Zhang Jie J Sun De-Hui DH Chu Yue-Feng YF Cai Xue-Peng XP Liu Xiang-Tao XT Luo Xue-Nong XN Liu Qing Q Liu Yong-Sheng YS
Journal of virological methods 20080319 2
A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the s ...[more]