Ontology highlight
ABSTRACT:
SUBMITTER: Zhang M
PROVIDER: S-EPMC7112847 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Zhang M M Yang J X JX Lin X M XM Zhu C H CH He J Q JQ Liu H H Lin T L TL
Journal of virological methods 20100423 2
A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle iridovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum. Using DAS-ELISA, the detection limit of STIV was found to be 10(3)PFU/ml. The positive rate of 15 STIV samples was 100%, while the positive rate of 100 other aquatic virus samples was 0%. These data show that DAS-ELISA is highly specific and sensitive for the detection ...[more]