Unknown

Dataset Information

0

A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens.


ABSTRACT: A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle iridovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum. Using DAS-ELISA, the detection limit of STIV was found to be 10(3)PFU/ml. The positive rate of 15 STIV samples was 100%, while the positive rate of 100 other aquatic virus samples was 0%. These data show that DAS-ELISA is highly specific and sensitive for the detection of STIV. In clinical tests, 128 samples isolated from pond-reared turtles were subjected to DAS-ELISA and PCR. The overall agreement between the results obtained by DAS-ELISA and PCR was 98.4%. The results indicate that the DAS-ELISA method could be used for diagnosing diseases caused by STIV.

SUBMITTER: Zhang M 

PROVIDER: S-EPMC7112847 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens.

Zhang M M   Yang J X JX   Lin X M XM   Zhu C H CH   He J Q JQ   Liu H H   Lin T L TL  

Journal of virological methods 20100423 2


A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle iridovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum. Using DAS-ELISA, the detection limit of STIV was found to be 10(3)PFU/ml. The positive rate of 15 STIV samples was 100%, while the positive rate of 100 other aquatic virus samples was 0%. These data show that DAS-ELISA is highly specific and sensitive for the detection  ...[more]

Similar Datasets

| S-EPMC9326611 | biostudies-literature
| S-EPMC7071587 | biostudies-literature
| S-EPMC7858105 | biostudies-literature
| S-EPMC2689277 | biostudies-literature
| S-EPMC7671975 | biostudies-literature
| S-EPMC3097095 | biostudies-literature
| S-EPMC7997747 | biostudies-literature
| S-EPMC5803225 | biostudies-literature
| S-EPMC2886893 | biostudies-other
| S-EPMC7649156 | biostudies-literature