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Controlled Synthesis of Manganese Oxide Nanoparticles Encaged in Hollow Mesoporous Silica Nanoreactors and Their Enhanced Dye Degradation Activity.

ABSTRACT: In this study, controlled synthesis of hollow mesoporous silica nanoreactors with small manganese oxide nanoparticles in their cavities (Mn x O y @HMSNs) is reported, and the dye degradation performance in the presence of hydrogen peroxide over Mn x O y @HMSNs is investigated. Specifically, triple ligands (a compound with three dipicolinic acid groups) were used to coordinate manganese ions to form negatively charged coordination complex networks, which further combine with positively charged copolymers to obtain metal ion-containing polymer micelles. Following silica deposition onto micellar coronas and calcinations simultaneously result in hollow mesoporous silica nanoreactors and manganese oxide nanoparticles in their cavities. In this work, the influences of synthetic parameters on the structures are studied in detail. The obtained Mn x O y @HMSNs show greatly enhanced activity and stability for a series of dye degradations. The performance enhancement is ascribed to their unique nanostructures, where mesoporous silica walls provide protection to the inner Mn x O y nanoparticles and the small size of the manganese oxide nanoparticles greatly enhances the dye degradation activity.

SUBMITTER: Wei J

PROVIDER: S-EPMC7114703 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Controlled Synthesis of Manganese Oxide Nanoparticles Encaged in Hollow Mesoporous Silica Nanoreactors and Their Enhanced Dye Degradation Activity.

Wei Jinxia J Li Kaijie K Yu Hongbo H Yin Hongfeng H Cohen Stuart Martien A MA Wang Junyou J Zhou Shenghu S

ACS omega 20200317 12

In this study, controlled synthesis of hollow mesoporous silica nanoreactors with small manganese oxide nanoparticles in their cavities (Mn x O y @HMSNs) is reported, and the dye degradation performance in the presence of hydrogen peroxide over Mn x O y @HMSNs is investigated. Specifically, triple ligands (a compound with three dipicolinic acid groups) were used to coordinate manganese ions to form negatively charged coordin ...[more]

PMID: 32258921

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Project description:Tunable glutathione (GSH)-sensitive hollow mesoporous silica nanoparticles (HMSiO2 NPs) were developed using a structural difference-based selective etching strategy. These organosilica hollow nanoparticles contained disulfide linkages (S-S) in the outer shell which were degraded by GSH. The particles were compared with their nonGSH-sensitive tetraethyl orthosilicate (TEOS) HMSiO2 counterparts in terms of their synthesis method, characterization, doxorubicin (DOX) release profile, and in vitro cytotoxicity in MCF-7 breast cancer cells. Transmission electron microscopy (TEM) of the particles indicated that the fabricated HMSiO2 NPs had an average diameter of 130?±?5?nm. Thermogravimetric analysis (TGA) revealed that GSH-sensitive particles had approximately 5.3% more weight loss than TEOS HMSiO2 NPs. Zeta potential of these redox-responsive particles was -23?±?1?mV at pH?6 in deionized (DI) water. Nitrogen adsorption-desorption isotherm revealed that the surface area of the hollow mesoporous nanoreservoirs was roughly 446?±?6?m2?g-1 and the average diameter of the pores was 2.3?±?0.5?nm. TEM images suggest that the nanoparticles started to lose mass integrity from Day 1. The particles showed a high loading capacity for DOX (8.9?±?0.5%) as a model drug, due to the large voids existing in the hollow structures. Approximately 58% of the incorporated DOX released within 14?days in phosphate buffered saline (PBS) at pH?6 and in the presence of 10?mM of GSH, mimicking intracellular tumor microenvironment while release from TEOS HMSiO2 NPs was only c.a. 18%. The uptake of these hollow nanospheres by MCF-7 cells and RAW 264.7 macrophages was evaluated using TEM and confocal microscopy. The nanospheres were shown to accumulate in the endolysosomal compartments after incubation for 24?h with the maximum uptake of c.a. 2.1?±?0.3% and 5.2?±?0.4%, respectively. Cytotoxicity of the nanospheres was investigated using CCK-8 assay. Results indicate that intact hollow particles (both GSH-sensitive and TEOS HMSiO2 NPs) were nontoxic to MCF-7 cells after incubation for 24?h within the concentration range of 0-1000??g?ml-1. DOX-loaded GSH-sensitive nanospheres containing 6??g?ml-1 of DOX killed c.a. 51% of MCF-7 cells after 24?h while TEOS HMSiO2 NPs killed c.a. 20% with the difference being statistically significant. Finally, cytotoxicity data in RAW 264.7 macrophages and NIH 3?T3 fibroblasts shows that intact GSH-sensitive HMSiO2 NPs did not show any toxic effects on these cells with the concentrations equal or <125??g?ml-1.

Project description:Simple Summary The combination of guanidine carbonate and curcumin-loaded hollow mesoporous silica nanoparticles (GuC-HMSNAP) can be used as a therapeutic and to induce MCF-7 cell death. Due to its biological safety and high drug loading capacity, HMSNAP is becoming an increasingly important nanocarrier for cancer research. The features of drug-loaded nanocarriers are significantly altered particle size, pore size, surface area, and pore volume, confirming that a significant amount of drugs can be loaded in the nanocarriers while simultaneously allowing the maximum amount of drugs to be released from the nanocarriers. Here, guanidine-mediated apoptosis is analysed through western blotting; the results suggest that drug complexes result in downregulation of phosphorylation in Ser471 of Akt, Ser259 of c-Raf, and Ser241 of PDK1, upregulation of phosphorylation in GSK-3β Ser9, cleaved caspases, and cleaved PARP, which then partially induces intrinsic cell death in MCF-7. As a whole, our results demonstrate that GuC-HMNSAP is an efficient nanocarrier for effectively inducing cancer cell death. Abstract The current study focuses on developing a tumour-targeted functionalised nanocarrier that wraps hollow mesoporous silica nanoparticles. The guanidine carbonate and curcumin are immobilised on the surface of 3-aminopropyl-triethoxy silane (APTES)-decorated hollow mesoporous silica nanoparticles (HMSNP), as confirmed through XPS and NMR analysis. XPS analysis demonstrates that the shape of the hysteresis loops is modified and that pore volume and pore diameter are consequently decreased compared to control. Guanidine (85%) and guanidine–curcumin complex (90%) were successfully encapsulated in HMSNAP and showed a 90% effective and sustained release at pH 7.4 for up to 72 h. Acridine orange/ethidium bromide dual staining determined that GuC-HMNSAP induced more late apoptosis and necrosis at 48 and 72 h compared with Gu-HMNSAP-treated cells. Molecular investigation of guanidine-mediated apoptosis was analysed using western blotting. It was found that cleaved caspases, c-PARP, and GSK-3β (Ser9) had increased activity in MCF-7 cells. GuC-HMSNAP increased the activity of phosphorylation of oncogenic proteins such as Akt (Ser473), c-Raf (Ser249), PDK1 (Ser241), PTEN (Ser380), and GSK-3β (Ser9), thus inducing cell death in MCF-7 cells. Altogether, our findings confirm that GuC-HMNSAP induces cell death by precisely associating with tumour-suppressing proteins, which may lead to new therapeutic approaches for breast cancer therapy.

Dataset Information

Controlled Synthesis of Manganese Oxide Nanoparticles Encaged in Hollow Mesoporous Silica Nanoreactors and Their Enhanced Dye Degradation Activity.

Publications

Controlled Synthesis of Manganese Oxide Nanoparticles Encaged in Hollow Mesoporous Silica Nanoreactors and Their Enhanced Dye Degradation Activity.

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