Dataset Information

Lipid metabolism of leukocytes in the unstimulated and activated states.

ABSTRACT: Lipidomics has emerged as a powerful technique to study cellular lipid metabolism. As the lipidome contains numerous isomeric and isobaric species resulting in a significant overlap between different lipid classes, cutting-edge analytical technology is necessary for a comprehensive analysis of lipid metabolism. Just recently, differential mobility spectrometry (DMS) has evolved as such a technology, helping to overcome several analytical challenges. We here set out to apply DMS and the Lipidyzer™ platform to obtain a comprehensive overview of leukocyte-related lipid metabolism in the resting and activated states. First, we tested the linearity and repeatability of the platform by using HL60 cells. We obtained good linearities for most of the thirteen analyzed lipid classes (correlation coefficient >?0.95), and good repeatability (%CV?

SUBMITTER: Alarcon-Barrera JC

PROVIDER: S-EPMC7118052 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Lipid metabolism of leukocytes in the unstimulated and activated states.

Alarcon-Barrera Juan Carlos JC von Hegedus Johannes H JH Brouwers Hilde H Steenvoorden Evelyne E Ioan-Facsinay Andreea A Mayboroda Oleg A OA Ondo-Mendez Alejandro A Giera Martin M

Analytical and bioanalytical chemistry 20200214 10

Lipidomics has emerged as a powerful technique to study cellular lipid metabolism. As the lipidome contains numerous isomeric and isobaric species resulting in a significant overlap between different lipid classes, cutting-edge analytical technology is necessary for a comprehensive analysis of lipid metabolism. Just recently, differential mobility spectrometry (DMS) has evolved as such a technology, helping to overcome several analytical challenges. We here set out to apply DMS and the Lipidyzer ...[more]

PMID: 32055910

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Project description:BackgroundMacrophage activation by lipopolysaccharide and adenosine triphosphate (ATP) has been studied extensively because this model system mimics the physiological context of bacterial infection and subsequent inflammatory responses. Previous studies on macrophages elucidated the biological roles of caspase-1 in post-translational activation of interleukin-1? and interleukin-18 in inflammation and apoptosis. However, the results from these studies focused only on a small number of factors. To better understand the host response, we have performed a high-throughput study of Kdo2-lipid A (KLA)-primed macrophages stimulated with ATP.ResultsThe study suggests that treating mouse bone marrow-derived macrophages with KLA and ATP produces 'synergistic' effects that are not seen with treatment of KLA or ATP alone. The synergistic regulation of genes related to immunity, apoptosis and lipid metabolism is observed in a time-dependent manner. The synergistic effects are produced by nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and activator protein (AP)-1 through regulation of their target cytokines. The synergistically regulated cytokines then activate signal transducer and activator of transcription (STAT) factors that result in enhanced immunity, apoptosis and lipid metabolism; STAT1 enhances immunity by promoting anti-microbial factors; and STAT3 contributes to downregulation of cell cycle and upregulation of apoptosis. STAT1 and STAT3 also regulate glycerolipid and eicosanoid metabolism, respectively. Further, western blot analysis for STAT1 and STAT3 showed that the changes in transcriptomic levels were consistent with their proteomic levels. In summary, this study shows the synergistic interaction between the toll-like receptor and purinergic receptor signaling during macrophage activation on bacterial infection.AvailabilityTime-course data of transcriptomics and lipidomics can be queried or downloaded from http://www.lipidmaps.org.Contactshankar@ucsd.edu.Supplementary informationSupplementary data are available at Bioinformatics online.

Dataset Information

Lipid metabolism of leukocytes in the unstimulated and activated states.

Publications

Lipid metabolism of leukocytes in the unstimulated and activated states.

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