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ABSTRACT:
SUBMITTER: Lemaitre E
PROVIDER: S-EPMC7119483 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Lemaitre E E Allée C C Vabret A A Eterradossi N N Brown P A PA
Journal of virological methods 20171010
Current molecular methods for the detection of avian and human metapneumovirus (AMPV, HMPV) are specifically targeted towards each virus species or individual subgroups of these. Here a broad range SYBR Green I real time RT-PCR was developed which amplified a highly conserved fragment of sequence in the N open reading frame. This method was sufficiently efficient and specific in detecting all MPVs. Its validation according to the NF U47-600 norm for the four AMPV subgroups estimated low limits o ...[more]