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Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein.

ABSTRACT: Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs at the atomic level. In this chapter, we describe the purification, crystallization, and structure determination of the MERS-CoV RBD.

SUBMITTER: Zhou H

PROVIDER: S-EPMC7123399 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein.

Zhou Haixia H Zhang Shuyuan S Wang Xinquan X

Methods in molecular biology (Clifton, N.J.) 20200101

Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs at the atomic level. In this chapter, we describe the purification, crystallization, and structure determination of the MERS-CoV RBD. ...[more]

PMID: 31883086

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