High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803.

ABSTRACT: Cyanobacteria and microalgae are attractive photoautotrophic host systems for climate-friendly production of fuels and other value-added biochemicals. However, for economic applications further development and implementation of efficient and sustainable cultivation strategies are essential. Here, we present a comparative study on cyanobacterial sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803 using a commercial lab-scale High Density Cultivation (HDC) platform in the presence of dodecane as in-situ extractant. Operating in a two-step semi-batch mode over a period of eight days, volumetric yields of (E)-α-bisabolene were more than two orders of magnitude higher than previously reported for cyanobacteria, with final titers of 179.4 ± 20.7 mg * L^-1. Likewise, yields of th

SUBMITTER: Dienst D 

PROVIDER: S-EPMC7125158 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature