Ontology highlight
ABSTRACT:
SUBMITTER: Rosen CB
PROVIDER: S-EPMC7125183 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Rosen Christian Bech CB Bayley Hagan H Rodriguez-Larrea David D
Communications biology 20200403 1
Protein post-translational translocation is found at the plasma membrane of prokaryotes and protein import into organellae. Translocon structures are becoming available, however the dynamics of proteins during membrane translocation remain largely obscure. Here we study, at the single-molecule level, the folding landscape of a model protein while forced to translocate a transmembrane pore. We use a DNA tag to drive the protein into the α-hemolysin pore under a quantifiable force produced by an a ...[more]