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Inositol Pyrophosphate Metabolism Regulates Presynaptic Vesicle Cycling at Central Synapses.


ABSTRACT: The coordination of synaptic vesicle exocytosis and endocytosis supports neurotransmitter release from presynaptic terminals. Although inositol pyrophosphates, such as 5-diphosphoinositol pentakisphosphate (5-IP7), are versatile signaling metabolites in many biological events, physiological actions of 5-IP7 on synaptic membrane vesicle trafficking remain unclear. Here, we investigated the role of 5-IP7 in synaptic transmission in hippocampal brain slices from inositol hexakisphosphate kinase 1 (Ip6k1)-knockout mice. We found that presynaptic release probability was significantly increased in Ip6k1-knockout neurons, implying enhanced activity-dependent synaptic vesicle exocytosis. Expression of wild-type but not catalytically inactive IP6K1 in the Ip6k1-knockout hippocampus restored the altered presynaptic release probability. Moreover, Ip6k1-knockout neurons were insensitive to folimycin, a vacuolar ATPase inhibitor, and dynasore, a dynamin inhibitor, suggesting marked impairment in synaptic endocytosis during exocytosis. Our findings collectively establish that IP6K1 and its product, 5-IP7, act as key physiological determinants for inhibition of presynaptic vesicle exocytosis and stimulation of endocytosis at central synapses.

SUBMITTER: Park SJ 

PROVIDER: S-EPMC7132149 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Inositol Pyrophosphate Metabolism Regulates Presynaptic Vesicle Cycling at Central Synapses.

Park Seung Ju SJ   Park Hoyong H   Kim Min-Gyu MG   Zhang Seungjae S   Park Seung Eun SE   Kim Seyun S   Chung ChiHye C  

iScience 20200322 4


The coordination of synaptic vesicle exocytosis and endocytosis supports neurotransmitter release from presynaptic terminals. Although inositol pyrophosphates, such as 5-diphosphoinositol pentakisphosphate (5-IP<sub>7</sub>), are versatile signaling metabolites in many biological events, physiological actions of 5-IP<sub>7</sub> on synaptic membrane vesicle trafficking remain unclear. Here, we investigated the role of 5-IP<sub>7</sub> in synaptic transmission in hippocampal brain slices from ino  ...[more]

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