Direct and Intervertebral Disc-Mediated Sensitization of Dorsal Root Ganglion Neurons by Hypoxia and Low pH.

ABSTRACT: OBJECTIVE:Ischemia-related risk factors are consistently correlated with discogenic pain, but it remains unclear how the ischemia-associated hypoxia and acidosis influence the peripheral sensory nervous system, namely the dorsal root ganglion (DRG), either directly or indirectly via intervertebral disc (IVD) mediation. METHODS:Bovine tail IVD organ cultures were preconditioned in different hypoxic and/or acidic conditions for 3 days to collect the conditioned medium (CM). The DRG-derived ND7/23 cells were either treated by the IVD CM or directly stimulated by hypoxic and/or acidic conditions. Neuronal sensitization was evaluated using calcium imaging (Fluo-4) after 3 days. RESULTS:We found that direct exposure of DRG cell line to hypoxia and acidosis increased both spontaneous and bradykin

SUBMITTER: Ma J 

PROVIDER: S-EPMC7136118 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature