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A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging.

ABSTRACT: Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.

SUBMITTER: Niekamp S

PROVIDER: S-EPMC7138518 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging.

Niekamp Stefan S Stuurman Nico N Vale Ronald D RD

Nature methods 20200316 4

Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision. ...[more]

PMID: 32203385

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