Dataset Information

Fibroblast Interaction with Different Abutment Surfaces: In Vitro Study.

ABSTRACT: BACKGROUND:Attaining an effective mucosal attachment to the transmucosal part of the implant could protect the peri-implant bone. AIM:To evaluate if chair side surface treatments (plasma of Argon and ultraviolet light) may affect fibroblast adhesion on different titanium surfaces designed for soft tissue healing. METHODS:Grade 5 titanium discs with four different surface topographies were subdivided into 3 groups: argon-plasma; ultraviolet light, and no treatment. Cell morphology and adhesion tests were performed at 20 min, 24 h, and 72 h. RESULTS:Qualitative observation of the surfaces performed at the SEM was in accordance with the anticipated features. Roughness values ranged from smooth (MAC Sa = 0.2) to very rough (XA Sa = 21). At 20 min, all the untreated surfaces presented hemispherical cells with reduced filopodia, while the cells on treated samples were more spread with broad lamellipodia. However, these differences in spreading behavior disappeared at 24 h and 72 h. Argon-plasma, but not UV, significantly increased the number of fibroblasts independently of the surface type but only at 20 min. Statistically, there was no surface in combination with a treatment that favored a greater cellular adhesion. CONCLUSIONS:Data showed potential biological benefits of treating implant abutment surfaces with the plasma of argon in relation to early-stage cell adhesion.

SUBMITTER: Canullo L

PROVIDER: S-EPMC7139398 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Fibroblast Interaction with Different Abutment Surfaces: In Vitro Study.

Canullo Luigi L Genova Tullio T Gross Trujillo Esperanza E Pradies Guillermo G Petrillo Sara S Muzzi Maurizio M Carossa Stefano S Mussano Federico F

International journal of molecular sciences 20200311 6

<h4>Background</h4>Attaining an effective mucosal attachment to the transmucosal part of the implant could protect the peri-implant bone.<h4>Aim</h4>To evaluate if chair side surface treatments (plasma of Argon and ultraviolet light) may affect fibroblast adhesion on different titanium surfaces designed for soft tissue healing.<h4>Methods</h4>Grade 5 titanium discs with four different surface topographies were subdivided into 3 groups: argon-plasma; ultraviolet light, and no treatment. Cell morp ...[more]

PMID: 32168919

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Project description:PurposeIn this prospective clinical pilot study, abutments with different topologies (machined versus polished) were compared with respect to the clinical outcome and the microbiological profile. Furthermore, three different sampling methods (retrieval of abutment, collection of peri-abutment exudate using paper-points, and a small peri-abutment soft-tissue biopsy) were evaluated for the identification and quantification of colonising bacteria.MethodsTwelve patients, seven with machined abutment and five with polished abutment, were included in the analysis. Three different sampling procedures were employed for the identification and quantification of colonising bacteria from baseline up to 12 months, using quantitative culturing. Clinical outcome measures (Holgers score, hygiene, pain, numbness and implant stability) were investigated.ResultsThe clinical parameters, and total viable bacteria per abutment or in tissue biopsies did not differ significantly between the polished and machined abutments. The total CFU/mm2 abutment and CFU/peri-abutment fluid space of anaerobes, aerobes and staphylococci were significantly higher for the polished abutment. Anaerobic bacteria were detected in the tissue biopsies before BAHS implantation. Anaerobes and Staphylococcus spp. were detected in all three compartments after BAHS installation. For most patients (10/12), the same staphylococcal species were found in at least two of the three compartments at the same time-point. The common skin coloniser Staphylococcus epidermidis was identified in all patients but one (11/12), whereas the pathogen Staphylococcus aureus was isolated in five of the patients. Several associations between clinical and microbiological parameters were found.ConclusionsThere was no difference in the clinical outcome with the use of polished versus machined abutment at 3 and 12 months after implantation. The present pilot trial largely confirmed a suitable study design, sampling and analytical methodology to determine the effects of modified BAHS abutment properties.Level of evidence2. Controlled prospective comparative study.

Dataset Information

Fibroblast Interaction with Different Abutment Surfaces: In Vitro Study.

Publications

Fibroblast Interaction with Different Abutment Surfaces: In Vitro Study.

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