The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism.

ABSTRACT: OBJECTIVE:Increasing evidence has revealed that mechanical stress and elevated mechanical signals promote malignant tumor transformation and metastasis. This study aimed to explore the function of the mechanically activated ion-channel Piezo1 in the colon cancer metastasis and its potential regulatory mechanism. METHODS:First, we examined the expression levels of Piezo1 and mitochondrial calcium uniporter (MCU) both in colon cancer tissues and assessed the prognostic value of Piezo1 and MCU in a colon cancer cohort (n?=?110). Second, functional assays were performed to investigate the effects of Piezo1 and MCU on colon cancer cell migration, invasion, and mitochondrial membrane potential. Third, we analyzed the expression of Piezo1, MCU, and HIF-1? by overexpressing/silencing each other's expression. RESULTS:We found that Piezo1 was up-regulated and MCU was down-regulated in colon cancer tissues. Piezo1 and MCU were both correlated with poor prognosis of patients with colon cancer. Overexpressing Piezo1 and silencing MCU could promote colon cancer cell migration and metastasis, reduce mitochondrial membrane potential, and promote each other's expression. We also found that HIF-1? was up-regulated in colon cancer tissues. Additionally, silencing Piezo1 inhibited the expression of HIF-1? and VEGF, which was contrary to MCU silencing. Intriguingly, Piezo1-overexpressing cells did not regain their migration behaviors when HIF-1? expression was inhibited, which was accompanied with the re-expression of MCU and VEGF. CONCLUSION:In our study, Piezo1 is involved in colon cancer cell metastasis. Furthermore, our findings indicated a possible Piezo1-MCU-HIF-1?-VEGF axis, which still need further exploration.

SUBMITTER: Sun Y 

PROVIDER: S-EPMC7142063 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature