Ontology highlight
ABSTRACT:
SUBMITTER: Tatiossian KJ
PROVIDER: S-EPMC7934447 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Tatiossian Kristina J KJ Clark Robert D E RDE Huang Chun C Thornton Matthew E ME Grubbs Brendan H BH Cannon Paula M PM
Molecular therapy : the journal of the American Society of Gene Therapy 20201014 3
Homology-directed repair (HDR) of a DNA break allows copying of genetic material from an exogenous DNA template and is frequently exploited in CRISPR-Cas9 genome editing. However, HDR is in competition with other DNA repair pathways, including non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ), and the efficiency of HDR outcomes is not predictable. Consequently, to optimize HDR editing, panels of CRISPR-Cas9 guide RNAs (gRNAs) and matched homology templates must be e ...[more]