Spectroscopic and Structural Analysis of Cu²⁺-Induced Fluorescence Quenching of ZsYellow.

ABSTRACT: Fluorescent proteins exhibit fluorescence quenching by specific transition metals, suggesting their potential as fluorescent protein-based metal biosensors. Each fluorescent protein exhibits unique spectroscopic properties and mechanisms for fluorescence quenching by metals. Therefore, the metal-induced fluorescence quenching analysis of various new fluorescent proteins would be important step towards the development of such fluorescent protein-based metal biosensors. Here, we first report the spectroscopic and structural analysis of the yellow fluorescent protein ZsYellow, following its metal-induced quenching. Spectroscopic analysis showed that ZsYellow exhibited a high degree of fluorescence quenching by Cu²⁺. During Cu²⁺-induced ZsYellow quenching, fluorescence em

SUBMITTER: Kim IJ 

PROVIDER: S-EPMC7146301 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature