Dataset Information

Foliar Pre-Treatment with Abscisic Acid Enhances Olive Tree Drought Adaptability.

ABSTRACT: Water is the most widely limiting factor for plants distribution, survival and agricultural productivity, their responses to drought and recovery being critical for their success and productivity. Olea europaea L. is a well-adapted species to cyclic drought events, still at considerable expense of carbon reserves and CO2 supply. To study the role of abscisic acid (ABA) as a promoter of drought adaptability, young potted olive trees subjected to three drought-recovery cycles were pre-treated with ABA. The results demonstrated that ABA pre-treatment allowed the delay of the drought effects on stomatal conductance (gs) and net photosynthesis (An), and under severe drought, permitted the reduction of the non-stomatal limitations to An and the relative water content decline, the accumulation of compatible solutes and avoid the decline of photosynthetic pigments, soluble proteins and total thiols concentrations and the accumulation of ROS. Upon rewatering, ABA-sprayed plants showed an early recovery of An. The plant ionome was also changed by the addition of ABA, with special influence on root K, N and B concentrations. The improved physiological and biochemical functions of the ABA-treated plants attenuated the drought-induced decline in biomass accumulation and potentiated root growth and whole-plant water use efficiency after successive drought-rewatering cycles. These changes are likely to be of real adaptive significance, with important implications for olive tree growth and productivity.

SUBMITTER: Brito C

PROVIDER: S-EPMC7154903 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Foliar Pre-Treatment with Abscisic Acid Enhances Olive Tree Drought Adaptability.

Brito Cátia C Dinis Lia-Tânia LT Ferreira Helena H Moutinho-Pereira José J Correia Carlos M CM

Plants (Basel, Switzerland) 20200308 3

Water is the most widely limiting factor for plants distribution, survival and agricultural productivity, their responses to drought and recovery being critical for their success and productivity. <i>Olea europaea</i> L. is a well-adapted species to cyclic drought events, still at considerable expense of carbon reserves and CO<sub>2</sub> supply. To study the role of abscisic acid (ABA) as a promoter of drought adaptability, young potted olive trees subjected to three drought-recovery cycles wer ...[more]

PMID: 32182702

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Project description:Susceptibility of plants to abiotic stresses, including extreme temperatures, salinity and drought, poses an increasing threat to crop productivity worldwide. Here the drought-induced response of maize was modulated by applications of methyl jasmonate (MeJA) and salicylic acid (SA) to seeds prior to sowing and to leaves prior to stress treatment. Pot experiments were conducted to ascertain the effects of exogenous applications of these hormones on maize growth, physiology and biochemistry under drought stress and well-watered (control) conditions. Maize plants were subjected to single as well as combined pre-treatments of MeJA and SA. Drought stress severely affected maize morphology and reduced relative water content, above and below-ground biomass, rates of photosynthesis, and protein content. The prolonged water deficit also led to increased relative membrane permeability and oxidative stress induced by the production of malondialdehyde (from lipid peroxidation), lipoxygenase activity (LOX) and the production of H2O2. The single applications of MeJA and SA were not found to be effective in maize for drought tolerance while the combined pre-treatments with exogenous MeJA+SA mitigated the adverse effects of drought-induced oxidative stress, as reflected in lower levels of lipid peroxidation, LOX activity and H2O2. The same pre-treatment also maintained adequate water status of the plants under drought stress by increasing osmolytes including proline, total carbohydrate content and total soluble sugars. Furthermore, exogenous applications of MeJA+SA approximately doubled the activities of the antioxidant enzymes catalase, peroxidase and superoxide dismutase. Pre-treatment with MeJA alone gave the highest increase in drought-induced production of endogenous abscisic acid (ABA). Pre-treatment with MeJA+SA partially prevented drought-induced oxidative stress by modulating levels of osmolytes and endogenous ABA, as well as the activities of antioxidant enzymes. Taken together, the results show that seed and foliar pre-treatments with exogenous MeJA and/or SA can have positive effects on the responses of maize seedlings to drought.

Project description:ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought stress. We identified barley TILLING mutants carrying different alleles in the HvABI5 gene and we studied in detail the physiological and molecular response to drought and ABA for one of them. The hvabi5.d mutant, carrying G1751A transition, was insensitive to ABA during seed germination, yet it showed the ability to store more water than its parent cv. "Sebastian" (WT) in response to drought stress. The drought-tolerant phenotype of hvabi5.d was associated with better membrane protection, higher flavonoid content, and faster stomatal closure in the mutant under stress compared to the WT. The microarray transcriptome analysis revealed up-regulation of genes associated with cell protection mechanisms in the mutant. Furthermore, HvABI5 target genes: HVA1 and HVA22 showed higher activity after drought, which may imply better adaptation of hvabi5.d to stress. On the other hand, chlorophyll content in hvabi5.d was lower than in WT, which was associated with decreased photosynthesis efficiency observed in the mutant after drought treatment. To verify that HvABI5 acts in the ABA-dependent manner we analyzed expression of selected genes related to ABA pathway in hvabi5.d and its WT parent after drought and ABA treatments. The expression of key genes involved in ABA metabolism and signaling differed in the mutant and the WT under stress. Drought-induced increase of expression of HvNCED1, HvBG8, HvSnRK2.1, and HvPP2C4 genes was 2-20 times higher in hvabi5.d compared to "Sebastian". We also observed a faster stomatal closure in hvabi5.d and much higher induction of HvNCED1 and HvSnRK2.1 genes after ABA treatment. Together, these findings demonstrate that HvABI5 plays a role in regulation of drought response in barley and suggest that HvABI5 might be engaged in the fine tuning of ABA signaling by a feedback regulation between biosynthetic and signaling events. In addition, they point to different mechanisms of HvABI5 action in regulating drought response and seed germination in barley.

Dataset Information

Foliar Pre-Treatment with Abscisic Acid Enhances Olive Tree Drought Adaptability.

Publications

Foliar Pre-Treatment with Abscisic Acid Enhances Olive Tree Drought Adaptability.

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