Ontology highlight
ABSTRACT:
SUBMITTER: Mo GCH
PROVIDER: S-EPMC7160135 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Nature communications 20200415 1
Genetically encoded Förster Resonance Energy Transfer (FRET)-based biosensors are powerful tools to illuminate spatiotemporal regulation of cell signaling in living cells, but the utility of the red spectrum for biosensing was limited due to a lack of bright and stable red fluorescent proteins. Here, we rationally improve the photophysical characteristics of the coral-derived fluorescent protein TagRFP-T. We show that a new single-residue mutant, super-TagRFP (stagRFP) has nearly twice the molec ...[more]