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C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors.

ABSTRACT: Hematopoietic stem cells (HSCs) and skeletal stem cells (SSCs) cohabit in the bone marrow. KITL (C-KIT ligand) from LEPR⁺ adult bone marrow stromal cells is pivotal for HSC maintenance. In contrast, it remains unclear whether KITL/C-KIT signaling also regulates SSCs. Here, we lineage traced C-KIT⁺ cells and found that C-KIT was expressed by fetal, but not postnatal skeletal progenitors. Fetal C-KIT⁺ cells gave rise to 20% of LEPR⁺ stromal cells in adult bone marrow, forming nearly half of all osteoblasts. Disruption of mTOR signaling in fetal C-KIT⁺ cells impaired bone formation. Notably, conditional deletion of Kitl from PRX1⁺ fetal bone marrow stromal cells, but not LEPR⁺ adult bone marrow stromal cells, significantly increased bone formation. Thus, our work identified C-KIT⁺ skeletal progenitors as an important source of bones formed during development.

SUBMITTER: He DD

PROVIDER: S-EPMC7160391 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors.

He Di Demi DD Tang Xinyu Thomas XT Dong Wenjie W Cui Guizhong G Peng Guangdun G Yin Xiujuan X Chen Yujie Y Jing Naihe N Zhou Bo O BO

Stem cell reports 20200326 4

Hematopoietic stem cells (HSCs) and skeletal stem cells (SSCs) cohabit in the bone marrow. KITL (C-KIT ligand) from LEPR+ adult bone marrow stromal cells is pivotal for HSC maintenance. In contrast, it remains unclear whether KITL/C-KIT signaling also regulates SSCs. Here, we lineage traced C-KIT+ cells and found that C-KIT was expressed by fetal, but not postnatal skeletal progenitors. Fetal C-KIT+ cells gave rise to 20% of LEPR+ stromal cells in adul ...[more]

PMID: 32220331

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