Dataset Information

Draft Genome Sequences of Two Acidophilic, Mesophilic Verrucomicrobial Methanotrophs Contain Only One pmoCAB Operon.

ABSTRACT: Methylacidimicrobium cyclopophantes 3B and Methylacidimicrobium tartarophylax 4AC are Gram-negative rod-shaped mesophilic methanotrophs isolated from soil samples with low pH at the Solfatara Crater, near Naples, Italy. The genomes of these extremophilic verrucomicrobia were sequenced using Illumina technology, and both species possess one pmoCAB operon and two xoxF genes.

SUBMITTER: Cremers G

PROVIDER: S-EPMC7163025 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Draft Genome Sequences of Two Acidophilic, Mesophilic Verrucomicrobial Methanotrophs Contain Only One pmoCAB Operon.

Cremers Geert G Pol Arjan A Jetten Mike S M MSM Op den Camp Huub J M HJM

Microbiology resource announcements 20200416 16

Methylacidimicrobium cyclopophantes 3B and Methylacidimicrobium tartarophylax 4AC are Gram-negative rod-shaped mesophilic methanotrophs isolated from soil samples with low pH at the Solfatara Crater, near Naples, Italy. The genomes of these extremophilic verrucomicrobia were sequenced using Illumina technology, and both species possess one pmoCAB operon and two xoxF genes. ...[more]

PMID: 32299887

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Project description:Genomic analysis of the methanotrophic verrucomicrobium "Methylacidiphilum infernorum" strain V4 has shown that most pathways conferring its methanotrophic lifestyle are similar to those found in proteobacterial methanotrophs. However, due to the large sequence divergence of its methane monooxygenase-encoding genes (pmo), "universal" pmoA polymerase chain reaction (PCR) primers do not target these bacteria. Unlike proteobacterial methanotrophs, "Methylacidiphilum" fixes carbon autotrophically, and uses methane only for energy generation. As a result, techniques used to detect methanotrophs in the environment such as (13)CH(4)-stable isotope probing (SIP) and pmoA-targeted PCR do not detect verrucomicrobial methanotrophs, and they may have been overlooked in previous environmental studies. We developed a modified SIP technique to identify active methanotrophic Verrucomicrobia in the environment by labeling with (13)CO(2) and (13)CH(4), individually and in combination. Testing the protocol in "M. infernorum" strain V4 resulted in assimilation of (13)CO(2) but not (13)CH(4), verifying its autotrophic lifestyle. To specifically detect methanotrophs (as opposed to other autotrophs) via (13)CO(2)-SIP, a quantitative PCR (qPCR) assay specific for verrucomicrobial-pmoA genes was developed and used in combination with SIP. Incubation of an acidic, high-temperature geothermal soil with (13)CH(4) + (12)CO(2) caused little shift in the density distribution of verrucomicrobial-pmoA genes relative to controls. However, labeling with (13)CO(2) in combination with (12)CH(4) or (13)CH(4) induced a strong shift in the distribution of verrucomicrobial-pmoA genes towards the heavy DNA fractions. The modified SIP technique demonstrated that the primary methanotrophs active in the soil were autotrophs and belonged to the Verrucomicrobia. This is the first demonstration of autotrophic, non-proteobacterial methanotrophy in situ, and provides a tool to detect verrucomicrobial methanotrophs in other ecosystems.

Dataset Information

Draft Genome Sequences of Two Acidophilic, Mesophilic Verrucomicrobial Methanotrophs Contain Only One pmoCAB Operon.

Publications

Draft Genome Sequences of Two Acidophilic, Mesophilic Verrucomicrobial Methanotrophs Contain Only One <i>pmoCAB</i> Operon.

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