Ontology highlight
ABSTRACT:
SUBMITTER: Sawasaki T
PROVIDER: S-EPMC7164004 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Sawasaki Tatsuya T Kamura Nami N Matsunaga Satoko S Saeki Mihoro M Tsuchimochi Masateru M Morishita Ryo R Endo Yaeta Y
FEBS letters 20071217 2
Protein microarray is considered to be one of the key analytical tools for high-throughput protein function analysis. Here, we report that the Arabidopsis HY5 functions as a novel DNA-binding tag (DBtag) for proteins. We also demonstrate that the DBtagged proteins could be immobilized and purified on a newly designed agarose/DNA microplate. Furthermore, we show three applications using the microarray: (1) detection of autophosphorylation activity of DBtagged human protein kinases and inhibition ...[more]