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Biosynthesis of Amino Acid Derived ?-Pyrones by an NRPS-NRPKS Hybrid Megasynthetase in Fungi.

ABSTRACT: A nonribosomal peptide synthetase (NRPS)-nonreducing polyketide synthase (NRPKS) hybrid enzyme (AnATPKS) from Aspergillus niger was shown to produce amino acid derived ?-pyrone natural products (pyrophen and campyrone B). Biochemical characterization of the NRPS module in vitro reveals that the adenylation domain is promiscuous toward a variety of substituted phenylalanine analogues. Using precursor feeding and heterologous expression of AnATPKS and an associated O-methyltransferase (AnOMT), we were able to access a library of substituted pyrophen analogues. Our study paves the way for future combinatorial biosynthesis of diverse ?-pyrone natural products using NRPS-NRPKS hybrids.

SUBMITTER: Hai Y

PROVIDER: S-EPMC7166049 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Biosynthesis of Amino Acid Derived α-Pyrones by an NRPS-NRPKS Hybrid Megasynthetase in Fungi.

Hai Yang Y Huang Arthur A Tang Yi Y

Journal of natural products 20200311 3

A nonribosomal peptide synthetase (NRPS)-nonreducing polyketide synthase (NRPKS) hybrid enzyme (AnATPKS) from Aspergillus niger was shown to produce amino acid derived α-pyrone natural products (pyrophen and campyrone B). Biochemical characterization of the NRPS module in vitro reveals that the adenylation domain is promiscuous toward a variety of substituted phenylalanine analogues. Using precursor feeding and heterologous expression of AnATPKS and an associated O-methyltransferas ...[more]

PMID: 32159958

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