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Age-related rhesus macaque models of COVID-19.

ABSTRACT: Background:Since December 2019, an outbreak of the Corona Virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, has become a public health emergency of international concern. The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models. Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2, and then analyzed by clinical signs, viral replication, chest X-ray, histopathological changes and immune response. Results:Viral replication of nasopharyngeal swabs, anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge. Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema, notably, old monkeys exhibited diffuse severe interstitial pneumonia. Viral antigens were detected mainly in alveolar epithelial cells and macrophages. Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys. Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection.

SUBMITTER: Yu P

PROVIDER: S-EPMC7167234 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Age-related rhesus macaque models of COVID-19.

Yu Pin P Qi Feifei F Xu Yanfeng Y Li Fengdi F Liu Peipei P Liu Jiayi J Bao Linlin L Deng Wei W Gao Hong H Xiang Zhiguang Z Xiao Chong C Lv Qi Q Gong Shuran S Liu Jiangning J Song Zhiqi Z Qu Yajin Y Xue Jing J Wei Qiang Q Liu Mingya M Wang Guanpeng G Wang Shunyi S Yu Haisheng H Liu Xing X Huang Baoying B Wang Wenling W Zhao Li L Wang Huijuan H Ye Fei F Zhou Weimin W Zhen Wei W Han Jun J Wu Guizhen G Jin Qi Q Wang Jianwei J Tan Wenjie W Qin Chuan C

Animal models and experimental medicine 20200330 1

<h4>Background</h4>Since December 2019, an outbreak of the Corona Virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, has become a public health emergency of international concern. The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.<h4>Methods</h4>Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-Co ...[more]

PMID: 32318665

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Project description:PurposeTo assess age-related changes in the rhesus macaque eye and evaluate them to corresponding human age-related eye disease.MethodsData from eye exams and imaging tests including intraocular pressure (IOP), lens thickness, axial length, and retinal optical coherence tomography (OCT) images were evaluated from 142 individuals and statistically analyzed for age-related changes. Quantitative autofluorescence (qAF) was measured as was the presence of macular lesions as related to age.ResultsAges of the 142 rhesus macaques ranged from 0.7 to 29 years (mean = 16.4 years, stdev = 7.5 years). Anterior segment measurements such as IOP, lens thickness, and axial length were acquired. Advanced retinal imaging in the form of optical coherence tomography and qAF were obtained. Quantitative assessments were made and variations by age groups were analyzed to compare with established age-related changes in human eyes. Quantitative analysis of data revealed age-related increase in intraocular pressure (0.165 mm Hg per increase in year of age), ocular biometry (lens thickness 7.2 μm per increase in year of age; and axial length 52.8 μm per increase in year of age), and presence of macular lesions. Age-related changes in thicknesses of retinal layers on OCT were observed and quantified, showing decreased thickness of the retinal ganglion cell layer and inner nuclear layer, and increased thickness of photoreceptor outer segment and choroidal layers. Age was correlated with increased qAF by 1.021 autofluorescence units per increase in year of age.ConclusionsThe rhesus macaque has age-related ocular changes similar to humans. IOP increases with age while retinal ganglion cell layer thickness decreases. Macular lesions develop in some aged animals. Our findings support the concept that rhesus macaques may be useful for the study of important age-related diseases such as glaucoma, macular diseases, and cone disorders, and for development of therapies for these diseases.

Dataset Information

Age-related rhesus macaque models of COVID-19.

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Age-related rhesus macaque models of COVID-19.

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