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Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.


ABSTRACT: Mesenchymal stem cells (MSCs) possess the ability to differentiate into multiple cell lineages, and thus, confer great potential for use in regenerative medicine and biotechnology. In the present study, we attempted to isolate and characterize bovine tongue tissue epithelium-derived MSCs (boT-MSCs) and investigate the culture conditions required for long-term culturing of boT-MSCs. boT-MSCs were successfully isolated by the collagenase digestion method and their proliferative capacity was maintained for up to 20 or more passages. We observed a significant increase in the proliferation of boT-MSCs during the 20 consecutive passages under low-glucose Dulbecco's modified Eagle's medium culture condition among the three culture conditions. These boT-MSCs presented pluripotency markers (octamer-binding transcription factor 3/4 (Oct3/4) and sex determining region Y-box2 (Sox2)) and cell surface markers, which included CD13, CD29, CD44, CD73, CD90, CD105, CD166, and major histocompatibility complex (MHC) class I (MHC-I) but not CD11b, CD14, CD31, CD34, CD45, CD80, CD86, CD106, CD117, and MHC-II at third passage. Moreover, these boT-MSCs could differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Thus, the present study suggests that the tongue of bovines could be used as a source of bovine MSCs.

SUBMITTER: Lee J 

PROVIDER: S-EPMC7167252 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.

Lee Jienny J   Byeon Jeong Su JS   Gu Na-Yeon NY   Lee Siu S   Lee Se-A SA   Jeong Da-Un DU   Ouh In-Ohk IO   Cho In-Soo IS   Song Jae-Young JY   Lee Yoon-Hee YH   Hyun Bang-Hun BH  

Bioscience reports 20200401 4


Mesenchymal stem cells (MSCs) possess the ability to differentiate into multiple cell lineages, and thus, confer great potential for use in regenerative medicine and biotechnology. In the present study, we attempted to isolate and characterize bovine tongue tissue epithelium-derived MSCs (boT-MSCs) and investigate the culture conditions required for long-term culturing of boT-MSCs. boT-MSCs were successfully isolated by the collagenase digestion method and their proliferative capacity was mainta  ...[more]

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