Workflow for Comparison of Chemical and Biological Metrics of Filter Collected PM2.5.
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ABSTRACT:
SUBMITTER: Roper C
PROVIDER: S-EPMC7170255 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:Toxicology research into the global public health burden of fine particulate matter (PM2.5) exposures frequently requires extraction of PM2.5 from filters. A standardized method for these extractions does not exist, leading to inaccurate interlaboratory comparisons. It is largely unknown how different filter extraction methods might impact the composition and bioactivity of the resulting samples. We characterized the variation in these metrics by using equal portions of a single PM2.5 filter, with each portion undergoing a different extraction method. Significant differences were observed between extraction methods for concentrations of elements and polycyclic aromatic hydrocarbons (PAHs) for the PM2.5 tested following its preparation for biological response studies. Importantly, the chemical profiles differed from those observed when we used standard protocols for chemical characterization of the ambient sample, demonstrating that extraction can alter both chemical component amounts and species profiles of the extracts. The impact of these chemical differences on sensitive end points of zebrafish development was investigated. Significant differences in the percent incidence and timing of mortality were associated with the PM2.5 extraction method. This research highlights the importance of and rationale for considering the extraction method when interlaboratory comparisons of PM2.5 toxicology research are made.
| S-EPMC6652177 | biostudies-literature
Project description:Fabrication of air filtration materials (AFM) that allow air to easily flow through while retaining particles is a significant and urgent need due to the harmful airborne particulate matter pollution; however, this is still a challenging research area. Herein, we report novel slip-effect functional nanofibrous membranes with decreased air resistance (reduction rate of 40%) due to the slip flow of air molecules on the periphery of nanofibers. This was achieved through careful control over the diameters of electrospun polyacrylonitrile fibers and aperture size of fiber assembly. Fiber assembly with 86% of fiber diameters between 60-100?nm was found to be most effective for slip flow, as these diameters are close to the mean free path of air molecules (65.3?nm). Significantly, an equilibrium factor ??=?df/d2 has been introduced to elucidate the effect of distance of adjacent fibers on the drag force of airflow. Furthermore, the most effective aperture size (>3.5??m) for slip-effect has been determined. Ultimately, the new material displayed low air resistance of 29.5?Pa, high purification efficiency of 99.09%, good transmittance of 77%, and long service life. The successful fabrication of such materials can facilitate the development of high-performance AFMs for various applications.
| S-EPMC5066256 | biostudies-literature
Project description:PM2.5 chemical components play significant roles in the climate, air quality, and public health, and the roles vary due to their different physicochemical properties. Obtaining accurate and timely updated information on China's PM2.5 chemical composition is the basis for research and environmental management. Here, we developed a full-coverage near-real-time PM2.5 chemical composition data set at 10 km spatial resolution since 2000, combining the Weather Research and Forecasting-Community Multiscale Air Quality modeling system, ground observations, a machine learning algorithm, and multisource-fusion PM2.5 data. PM2.5 chemical components in our data set are in good agreement with the available observations (correlation coefficients range from 0.64 to 0.75 at a monthly scale from 2000 to 2020 and from 0.67 to 0.80 at a daily scale from 2013 to 2020; most normalized mean biases within ±20%). Our data set reveals the long-term trends in PM2.5 chemical composition in China, especially the rapid decreases after 2013 for sulfate, nitrate, ammonium, organic matter, and black carbon, at the rate of -9.0, -7.2, -8.1, -8.4, and -9.2% per year, respectively. The day-to-day variability is also well captured, including evolutions in spatial distribution and shares of PM2.5 components. As part of Tracking Air Pollution in China (http://tapdata.org.cn), this daily-updated data set provides large opportunities for health and climate research as well as policy-making in China.
| S-EPMC9670839 | biostudies-literature
Project description:Greater Cairo (Egypt) is a megalopolis where the studies of the air pollution events are of extremely high relevance, for the geographical-climatological aspects, the anthropogenic emissions and the health impact. While preliminary studies on the particulate matter (PM) chemical composition in Greater Cairo have been performed, no data are yet available on the PM's toxicity. In this work, the in vitro toxicity of the fine PM (PM2.5) sampled in an urban area of Greater Cairo during 2017-2018 was studied. The PM2.5 samples collected during spring, summer, autumn and winter were preliminary characterized to determine the concentrations of ionic species, elements and organic PM (Polycyclic Aromatic Hydrocarbons, PAHs). After particle extraction from filters, the cytotoxic and pro-inflammatory effects were evaluated in human lung A549 cells. The results showed that particles collected during the colder seasons mainly induced the xenobiotic metabolizing system and the consequent antioxidant and pro-inflammatory cytokine release responses. Biological events positively correlated to PAHs and metals representative of a combustion-derived pollution. PM2.5 from the warmer seasons displayed a direct effect on cell cycle progression, suggesting possible genotoxic effects. In conclusion, a correlation between the biological effects and PM2.5 physico-chemical properties in the area of study might be useful for planning future strategies aiming to improve air quality and lower health hazards.
| S-EPMC6829270 | biostudies-literature
Project description:Observations of air pollution in Krakow have shown that air quality has been improved during the last decade. In the presented study two factors affecting the physicochemical characteristic of PM2.5 fraction at AGH station in Krakow were observed. One is the ban of using solid fuels for heating purposes and the second is COVID-19 pandemic in Krakow. The PM2.5 fraction was collected during the whole year every 3rd day between 2nd March 2020 and 28th February 2021 at AGH station in Krakow. In total 110 PM2.5 fraction samples were collected. The chemical composition was determined for these samples. The elemental analysis was performed by energy dispersive X-ray fluorescence (EDXRF) technique, ions analysis was performed by ion chromatography (IC) and black carbon by optical method. In order to identify the emission sources the positive matrix factorization (PMF) was used. The results of such study were compared to similar analysis performed for PM2.5 for the period from June 2018 to May 2019 at AGH station in Krakow. The PM2.5 concentration dropped by 25% in 2020/2021 in comparison to 2018/2019 at this station. The concentrations of Si, K, Fe, Zn and Pb were lowering by 43-64% in the year 2020/2021 in comparison to 2018/2019. Cu, Mn, Zn and Pb come from mechanical abrasion of brakes and tires while Ti, Fe, Mn and Si are crustal species. They are the indicators of road dust (non-exhaust traffic source). Moreover, the annual average contribution of traffic/industrial/soil/construction work source was reduced in 2020/2021 in comparison to 2018/2019. As well the annual average contribution of fuels combustion was declining by 22% in 2020/2021 in comparison to 2018/2019. This study shows that the ban and lockdown, during COVID-19 pandemic, had significant impact on the characteristic of air pollution in Krakow.
| S-EPMC9526202 | biostudies-literature
Project description:Solvent extraction of PM2.5 samples collected on the filter is a preliminary step for assessing the PM2.5 oxidative potential (OP) using cell-free assays, as the dithiothreitol (DTT) and the ascorbic acid (AA) assays. In this study, we evaluated the effect of the solvent choice by extracting ambient PM2.5 samples with different solvents: methanol, as organic solvent, and two aqueous buffers, i.e., phosphate buffer (PB) and Gamble's solution (G), as a lung fluid surrogate solution. Both the measured volume-based OPVDTT and OPVAA responses varied for the different extraction methods, since methanol extraction generated the lowest values and phosphate buffer the highest. Although all the tested solvents produced intercorrelated OPVDTT values, the phosphate buffer resulted the most useful for OPDTT assessment, as it provided the most sensible measure (nearly double values) compared with other extractions. The association of the measured OPV values with PM chemical composition suggested that oxidative properties of the investigated PM2.5 samples depend on both transition metals and quinones, as also supported by additional experimental measurements on standard solutions of redox-active species.
| S-EPMC8222022 | biostudies-literature
Project description:Water-soluble and trace metal species in fine particulate matter (PM2.5) were determined for indoor and outdoor environments in Doha, Qatar. During the study period, PM2.5 concentrations showed significant variability across several indoor locations ranging from 7.1 to 75.8 μg m-3, while the outdoor mass concentration range was 34.7-154.4 µg m-3. The indoor and outdoor PM2.5 levels did not exhibit statistically significant correlation, suggesting efficient building envelope protection against outdoor PM2.5 pollution. Rather than outdoor sources, human activities such as cooking, cleaning, and smoking were the most significant influence on chemical composition of indoor PM2.5. NH4+ concentration was insufficient to neutralize SO42- indoors and outdoors, indicating the predominant presence of NH4HSO4. The enrichment factors indicated that outdoor Fe, Mn, Co, Cr, and Ni in PM2.5 mostly originated from crustal sources. In contrast, the remaining outdoor trace metals (Cu, Zn, As, Cd, Pb, and V) were mainly derived from anthropogenic sources. The indoor/outdoor concentration ratios revealed significant indoor sources for NH4+ and Cu. The crustal matter, water-soluble ions, and sea salt explained 42%, 21%, and 1% of the indoor PM2.5 mass, respectively. The same groups sequentially constituted 41%, 16%, and 1% of the outdoor PM2.5 mass.
| S-EPMC11324777 | biostudies-literature
Project description:Personal exposure PM samples aid in determining the sources and chemical composition of real-world exposures, particularly in settings with household air pollution. However, their use in toxicological research is limited, despite uncertainty regarding health effects in these settings and evidence of differential toxicity among PM2.5 sources and components. This study used women's PM2.5 exposure samples collected using personal exposure monitoring in rural villages in three Chinese provinces (Beijing, Shanxi, and Sichuan) during summer and winter. Water-soluble organic carbon, ions, elements, and organic tracers (e.g. levoglucosan and polycyclic aromatic hydrocarbons [PAHs]) were quantified in water and organic PM2.5 extracts. Human lung epithelial cells (A549) were exposed to the extracts. Cell death, reactive oxygen species (ROS), and gene expression were measured. Biomass burning contributions were higher in Sichuan samples than in Beijing or Shanxi. Some PM characteristics (total PAHs and coal combustion source contributions) and biological effects of organic extract exposures (cell death, ROS, and cytokine gene expression) shared a common trend of higher levels and effects in winter than in summer for Shanxi and Beijing but no seasonal differences in Sichuan. Modulation of phase I/AhR-related genes (cyp1a1 and cyp1b1) and phase II/oxidative stress-related genes (HO-1, SOD1/2, NQO-1, and catalase) was either low or insignificant, without clear trends between samples. No significant cell death or ROS production was observed for water extract treatments among all sites and seasons, even at possible higher concentrations tested. These results support organic components, particularly PAHs, as essential drivers of biological effects, which is consistent with some other evidence from ambient PM2.5.
| S-EPMC8459644 | biostudies-literature
Project description:IntroductionDespite the London Underground (LU) handling on average 2.8 million passenger journeys per day, the characteristics and potential health effects of the elevated concentrations of metal-rich PM2.5 found in this subway system are not well understood.MethodsSpatial monitoring campaigns were carried out to characterise the health-relevant chemical and physical properties of PM2.5 across the LU network, including diurnal and day-to-day variability and spatial distribution (above ground, depth below ground and subway line). Population-weighted station PM2.5 rankings were produced to understand the relative importance of concentrations at different stations and on different lines.ResultsThe PM2.5 mass in the LU (mean 88??g?m-3, median 28??g?m-3) was greater than at ambient background locations (mean 19??g?m-3, median 14??g?m-3) and roadside environments in central London (mean 22??g?m-3, median 14??g?m-3). Concentrations varied between lines and locations, with the deepest and shallowest submerged lines being the District (median 4??g?m-3) and Victoria (median 361??g?m-3 but up to 885??g?m-3). Broadly in agreement with other subway systems around the world, sampled LU PM2.5 comprised 47% iron oxide, 7% elemental carbon, 11% organic carbon, and 14% metallic and mineral oxides. Although a relationship between line depth and air quality inside the tube trains was evident, there were clear influences relating to the distance from cleaner outside air and the exchange with cabin air when the doors open. The passenger population-weighted exposure analysis demonstrated a method to identify stations that should be prioritised for remediation to improve air quality.ConclusionPM2.5 concentrations in the LU are many times higher than in other London transport Environments. Failure to include this environment in epidemiological studies of the relationship between PM2.5 and health in London is therefore likely to lead to a large exposure misclassification error. Given the significant contribution of underground PM2.5 to daily exposure, and the differences in composition compared to urban PM2.5, there is a clear need for well-designed studies to better understand the health effects of underground exposure.
| S-EPMC6902242 | biostudies-literature
Project description:PM2.5 in the indoor and outdoor environment has been linked in epidemiology studies to the symptoms, hospital admissions and development of numerous health outcomes including death. The study was conducted during April 2017 and April 2018. PM2.5 samples were collected over 24 h and every third day. The mean PM2.5 level was 13.4 ?g m-3 (range: 1.17-39.1 ?g m-3). PM2.5 levels exceeded the daily World Health Organization air quality guideline (25 ?g m-3) on 14 occasions. The mean soot level was 1.38 m-1?×?10-5 (range: 0 to 5.38 m-1?×?10-5). Cl-, NO3 -, SO4 2-, Al, Ca, Fe, Mg, Na and Zn were detected in the PM2.5 samples. The geographical origin of air masses that passed Cape Town was estimated using the Hybrid Single Particle Lagrangian Integrated Trajectory software. Four air masses were identified in the cluster analysis: Atlantic-Ocean-WSW, Atlantic-Ocean-SW, Atlantic-Ocean-SSW and Indian-Ocean. The population of Cape Town may experience various health outcomes from the outdoor exposure to PM2.5 and the chemical composition of PM2.5.
| S-EPMC7539287 | biostudies-literature