Ontology highlight
ABSTRACT:
SUBMITTER: Overall SA
PROVIDER: S-EPMC7170893 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Overall Sarah A SA Toor Jugmohit S JS Hao Stephanie S Yarmarkovich Mark M Sara M O'Rourke Morozov Giora I GI Nguyen Son S Japp Alberto Sada AS Gonzalez Nicolas N Moschidi Danai D Betts Michael R MR Maris John M JM Smibert Peter P Sgourakis Nikolaos G NG
Nature communications 20200420 1
Peptide exchange technologies are essential for the generation of pMHC-multimer libraries used to probe diverse, polyclonal TCR repertoires in various settings. Here, using the molecular chaperone TAPBPR, we develop a robust method for the capture of stable, empty MHC-I molecules comprising murine H2 and human HLA alleles, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. Alternatively, catalytic amounts of TAPBPR can be used to exchange placeholde ...[more]