Ontology highlight
ABSTRACT:
SUBMITTER: Liu J
PROVIDER: S-EPMC7172788 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Liu Jianbo J Shi Hongyan H Chen Jianfei J Zhang Xin X Ji Zhaoyang Z Yuan Jing J Shi Da D Cao Liyan L Zhu Xiangdong X Dong Hui H Wang Xiaobo X Zhang Jialin J Feng Li L
Virology 20170429
Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutrali ...[more]