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Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody.


ABSTRACT: Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutralized new variant genotype 2 PEDV strains (LNCT2, LNSY, and Hjms), but it did not neutralize a genotype 1 PEDV strain (CV777), in vitro. Results showed that the epitope recognized by the 1B9 mAb lies in the spike protein, and that it is a conformational epitope. These findings confirm that allelic differences in the PEDV S gene between the G1 and G2 genotype strains led to changes in the S protein and, thus, differences in its immunogenicity.

SUBMITTER: Liu J 

PROVIDER: S-EPMC7172788 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody.

Liu Jianbo J   Shi Hongyan H   Chen Jianfei J   Zhang Xin X   Ji Zhaoyang Z   Yuan Jing J   Shi Da D   Cao Liyan L   Zhu Xiangdong X   Dong Hui H   Wang Xiaobo X   Zhang Jialin J   Feng Li L  

Virology 20170429


Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutrali  ...[more]

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