Matrix-Matched Calibration Curves for Assessing Analytical Figures of Merit in Quantitative Proteomics.
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ABSTRACT:
SUBMITTER: Pino LK
PROVIDER: S-EPMC7175947 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
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Matrix-Matched Calibration Curves for Assessing Analytical Figures of Merit in Quantitative Proteomics.
Pino Lindsay K LK Searle Brian C BC Yang Han-Yin HY Hoofnagle Andrew N AN Noble William S WS MacCoss Michael J MJ
Journal of proteome research 20200224 3
Mass spectrometry is a powerful tool for quantifying protein abundance in complex samples. Advances in sample preparation and the development of data-independent acquisition (DIA) mass spectrometry approaches have increased the number of peptides and proteins measured per sample. Here, we present a series of experiments demonstrating how to assess whether a peptide measurement is quantitative by mass spectrometry. Our results demonstrate that increasing the number of detected peptides in a prote ...[more]
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