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A cell-based assay for the detection of neutralizing antibodies against alemtuzumab.

ABSTRACT: Aim: The humanized anti-CD52 monoclonal antibody alemtuzumab depletes lymphocytes and is currently used to treat relapsing multiple sclerosis. During treatment, anti-alemtuzumab antibodies may develop and reduce effective lymphocyte depletion in future treatment cycles. Results: Alemtuzumab-Alexa Fluor 488 conjugate binding to the CHO-CD52 cell surface was inhibited by anti-alemtuzumab antibodies. Conclusion: In this proof-of-concept study, a CHO-CD52 cell line has been developed and used to detect the presence of anti-alemtuzumab neutralizing antibodies. This platform provides the basis of an assay for routine screening of serum for neutralizing antibodies from patients treated with alemtuzumab.

SUBMITTER: Ali L

PROVIDER: S-EPMC7177201 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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A cell-based assay for the detection of neutralizing antibodies against alemtuzumab.

Ali Liaqat L Saxena Gauri G Jones Meleri M Leisegang Georgia R GR Gammon Luke L Gnanapavan Sharmilee S Giovannoni Gavin G Schmierer Klaus K Baker David D Kang Angray S AS

BioTechniques 20200225 4

Aim: The humanized anti-CD52 monoclonal antibody alemtuzumab depletes lymphocytes and is currently used to treat relapsing multiple sclerosis. During treatment, anti-alemtuzumab antibodies may develop and reduce effective lymphocyte depletion in future treatment cycles. Results: Alemtuzumab-Alexa Fluor 488 conjugate binding to the CHO-CD52 cell surface was inhibited by anti-alemtuzumab antibodies. Conclusion: In this proof-of-concept study, a CHO-CD52 cell line has been deve ...[more]

PMID: 32096651

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