Dataset Information

Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device.

ABSTRACT: Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based microfluidic platform for colorimetric profiling of miRNAs, without the use of complex external equipment for fluidics and imaging. For sensitive and reliable measurement without the risk of sequence bias, we employed a gold deposition-based signal amplification scheme and dark-field imaging, and seamlessly integrated a previously developed miRNA assay scheme into this platform. The assay demonstrated a limit of detection of 260 fM, along with multiplexing of small panels of miRNAs in healthy and cancer samples. We anticipate this versatile platform to facilitate a broad range of POC profiling of miRNAs in cancer-associated dysregulation with high-confidence by exploiting the unique features of hydrogel substrate in an on-chip format and colorimetric analysis.

SUBMITTER: Lee H

PROVIDER: S-EPMC7178251 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device.

Lee Hyewon H Lee Jiseok J Lee Seung-Goo SG Doyle Patrick S PS

Analytical chemistry 20200402 8

Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based microfluidic platform for colorimetric profiling of miRNAs, without the use of complex external equipment for fluidics and imaging. For sensitive and reliable measurement without the risk of sequence ...[more]

PMID: 32207967

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Project description:Arsenic (As) as a metal ion has long-term toxicity and its presence in water poses a serious threat to the environment and human health. So, rapid and accurate recognition of traces of As is of particular importance in environmental and natural resources. In this study, a fast and sensitive colorimetric method was developed using silver nano prisms (Ag NPrs), cysteine-capped Ag NPrs, and methionine-capped Ag NPrs for accurate detection of arsenic-based on transforming the morphology of silver nanoparticles (AgNPs). The generated Ag atoms from the redox reaction of silver nitrate and As(iii) were deposited on the surface of Ag NPrs and their morphology changed to a circle. The morphological changes resulted in a change in the color of the nanoparticles from blue to purple, which was detectable by the naked eye. The rate of change was proportional to the concentration of arsenic. The changes were also confirmed using UV-Vis absorption spectra and showed a linear relationship between the change in adsorption peak and the concentration of arsenic in the range of 0.0005 to 1 ppm with a lower limit of quantification (LLOQ) of 0.0005 ppm. The proposed probes were successfully used to determine the amount of As(iii) in human urine samples. In addition, modified microfluidic substrates were fabricated with Ag NPrs, Cys-capped Ag NPrs, and methionine-capped Ag NPrs nanoparticles that are capable of arsenic detection in the long-time and can be used in the development of on-site As(iii) detection kits. In addition, silver nanowires (AgNWs) were used as a probe to detect arsenic, but good results were not obtained in human urine specimens and paper microfluidic platforms. In this study, for the first time, AgNPs were developed for optical colorimetric detection of arsenic using paper-based microfluidics. Ag NPrs performed best in both optical and colorimetric techniques. Therefore, they can be a promising option for the development of sensitive, inexpensive, and portable tools in the environmental and biomedical diagnosis of As(iii).

Dataset Information

Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device.

Publications

Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device.

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