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Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender.

ABSTRACT: Lavender (Lavandula angustifolia) is an important economic plant because of the value of its essential oil (EO). The Yili Valley in Xinjiang has become the largest lavender planting base in China. However, there is a lack of research on the gene expression regulation of EO biosynthesis and metabolism in local varieties. Here, de novo transcriptome analysis of inflorescence of three development stages from initial flower bud to flowering stage 50% from two lavender cultivars with contrasting EO production revealed the dynamics of 100,177 differentially expressed transcripts (DETs) in various stages of spike development within and across the cultivars. The lavender transcriptome contained 77 DETs with annotations related to terpenoid biosynthesis. The expression profiles of the 27 genes involved in the methylerythritol phosphate (MEP) pathway, 22 genes in the mevalonate (MVA) pathway, 28 genes related to monoterpene and sesquiterpene biosynthesis during inflorescence development were comprehensively characterized, and possible links between the expression changes of genes and contents of EO constituents were explored. The upregulated genes were mainly concentrated in the MEP pathway, while most genes in the MVA pathway were downregulated during flower development, and cultivars with a higher EO content presented higher expression of genes in the MEP pathway, indicating that EOs were chiefly produced through the MEP pathway. Additionally, MYB transcription factors constituted the largest number of transcripts in all samples, suggesting their potential roles in regulating EO biosynthesis. The sequences and transcriptional patterns of the transcripts will be helpful for understanding the molecular basis of lavender terpene biosynthesis.

SUBMITTER: Guo D

PROVIDER: S-EPMC7181790 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender.

Guo Danli D Kang Kaicheng K Wang Pu P Li Min M Huang Xianzhong X

Scientific reports 20200424 1

Lavender (Lavandula angustifolia) is an important economic plant because of the value of its essential oil (EO). The Yili Valley in Xinjiang has become the largest lavender planting base in China. However, there is a lack of research on the gene expression regulation of EO biosynthesis and metabolism in local varieties. Here, de novo transcriptome analysis of inflorescence of three development stages from initial flower bud to flowering stage 50% from two lavender cultivars with contrasting EO p ...[more]

PMID: 32332830

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Project description:BackgroundRose-scented geranium (Pelargonium sp.) is a perennial herb that produces a high value essential oil of fragrant significance due to the characteristic compositional blend of rose-oxide and acyclic monoterpenoids in foliage. Recently, the plant has also been shown to produce tartaric acid in leaf tissues. Rose-scented geranium represents top-tier cash crop in terms of economic returns and significance of the plant and plant products. However, there has hardly been any study on its metabolism and functional genomics, nor any genomic expression dataset resource is available in public domain. Therefore, to begin the gains in molecular understanding of specialized metabolic pathways of the plant, de novo sequencing of rose-scented geranium leaf transcriptome, transcript assembly, annotation, expression profiling as well as their validation were carried out.ResultsDe novo transcriptome analysis resulted a total of 78,943 unique contigs (average length: 623 bp, and N50 length: 752 bp) from 15.44 million high quality raw reads. In silico functional annotation led to the identification of several putative genes representing terpene, ascorbic acid and tartaric acid biosynthetic pathways, hormone metabolism, and transcription factors. Additionally, a total of 6,040 simple sequence repeat (SSR) motifs were identified in 6.8% of the expressed transcripts. The highest frequency of SSR was of tri-nucleotides (50%). Further, transcriptome assembly was validated for randomly selected putative genes by standard PCR-based approach. In silico expression profile of assembled contigs were validated by real-time PCR analysis of selected transcripts.ConclusionBeing the first report on transcriptome analysis of rose-scented geranium the data sets and the leads and directions reflected in this investigation will serve as a foundation for pursuing and understanding molecular aspects of its biology, and specialized metabolic pathways, metabolic engineering, genetic diversity as well as molecular breeding.

Project description:BackgroundThe anthocyanins are highly enriched in eggplants (Solanum melongena L.) with purple peel. However, our previous study showed that anthocyanins biosynthesis in eggplant cultivar 'Lanshan Hexian' was completely regulated by light and color becomes evident at most 2 days after exposure to light. In the present investigation, transcriptome study was made to explore the underlying molecular mechanisms of light-induced anthocyanin biosynthesis in eggplant (Solanum melongena L.) before color becomes evident.ResultsRNA-Seq was performed for four time points (0, 0.5, 4 and 8 h after bags removal) where concerted changes happened. A total of 32,630 genes or transcripts were obtained by transcriptome sequencing, from which 1956 differentially expressed genes (DEGs) were found. Gene Ontology analysis showed that the 1956 DEGs covered a wide range of cellular components, molecular functions and biological processes. All the DEGs were further divided into 26 clusters based on their distinct expression patterns. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis found out 24 structural anthocyanin biosynthesis genes which distributing in seven clusters. In addition, 102 transcription factors, which exhibited highly dynamic changes in response to light, were found in the seven clusters. Three photoreceptors, UV Resistance Locus 8 (UVR8), Cryptochrome 3 (CRY3) and UVR3, were identified as DEGs. The light signal transduction elements, COP1 and two SPAs, might be responsible for anthocyanin biosynthesis regulation.ConclusionBased on the transcriptome data, the anthocyanin biosynthesis structural genes, transcription factors, photoreceptors and light signal transduction elements were quickly screened which may act as the key regulatory factors in anthocyanin biosynthesis pathway. By comparing the transcriptome data with our previous studies, 869 genes were confirmed to participate in the light-induced anthocyanin biosynthesis. These results expand our knowledge of light-induced anthocyanin biosynthesis in plants, which allowing for fruit coloration to be improved under low-light conditions in future.

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Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender.

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Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender.

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