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ABSTRACT:
SUBMITTER: Guin D
PROVIDER: S-EPMC7183356 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Guin Drishti D Mittal Shriyaa S Bozymski Brian B Shukla Diwakar D Gruebele Martin M
The journal of physical chemistry letters 20190508 10
Denaturants such as the guanidinium cation unfold proteins at molar concentrations, which interferes with ultraviolet- and infrared-based spectroscopy measurements. Dodine denatures some proteins cooperatively at a thousand-fold lower concentration, allowing for spectroscopy measurements. Nonetheless, dodine's microscopic mechanism of interaction with proteins is not understood. We probe the effect of dodine on α-helices and tertiary structure by investigating the stability of the small helical ...[more]