Dataset Information

Serotypes, virulence genes and polymorphism of capsule gene cluster in Lactococcus garvieae isolated from diseased rainbow trout (Oncorhynchus mykiss) and mugger crocodile (Crocodylus palustris) in Iran.

ABSTRACT: Background:Lactococcus garvieae causes lactococcosis in rainbow trout in many parts of the world. Aims:This study was conducted for the existence of the virulent factors and differentiation of the two serotypes in L. garvieae. Methods:Twenty-two strains of L. garvieae isolated from diseased rainbow trout from farms in different regions and mugger crocodile of Iran, were investigated. In order to rapidly detect the presence of the hly1, hly2, hly3, NADH oxidase, sod, pgm, adhPsaA, eno, LPxTG-3, adhCI, and adhCII virulence genes, two multiplex polymerase chain reaction (PCR) assays were developed. Also, simplex PCR method was used to identify the bacterial serotypes, CGC, LPxTG-2, Adhesion, and adhPav virulence genes using the specific primer. Results:All varieties of L. garvieae contained the hly1, hly2, hly3, NADH oxidase, pgm, adhPav, LPxTG-3, sod, eno, adhPsaA, adhCI, and CGC virulence genes. Also, adhCII gene was present in all strains except one of the isolates originated from mugger crocodile. In addition, LPxTG-2 gene was only present in one of the isolates belonging to mugger crocodile. Adhesion gene was not present in all the strains. Interestingly, all the 22 strains originated from both hosts were identified as belonging to the serotype I. Based on the phylogenetic sequences of the capsule gene cluster, group all fish isolates into a cluster together with one isolate obtained from mugger crocodile. Conclusion:Further studies are recommended to investigate the role of virulence genes in L. garvieae and evaluate their pathogenicity to rainbow trout.

SUBMITTER: Salighehzadeh R

PROVIDER: S-EPMC7183378 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Serotypes, virulence genes and polymorphism of capsule gene cluster in <i>Lactococcus garvieae</i> isolated from diseased rainbow trout (<i>Oncorhynchus</i> <i>mykiss</i>) and mugger crocodile (<i>Crocodylus</i> <i>palustris</i>) in Iran.

Salighehzadeh R R Sharifiyazdi H H Akhlaghi M M Soltanian S S

Iranian journal of veterinary research 20200101 1

<h4>Background</h4><i>Lactococcus garvieae</i> causes lactococcosis in rainbow trout in many parts of the world.<h4>Aims</h4>This study was conducted for the existence of the virulent factors and differentiation of the two serotypes in <i>L. garvieae</i>.<h4>Methods</h4>Twenty-two strains of <i>L. garvieae</i> isolated from diseased rainbow trout from farms in different regions and mugger crocodile of Iran, were investigated. In order to rapidly detect the presence of the <i>hly1</i>, <i>hly2</i ...[more]

PMID: 32368222

Similar Datasets

Project description:BACKGROUND:Maternal transcripts are accumulated in the oocyte during oogenesis to provide for protein synthesis from oocyte maturation through early embryonic development, when nuclear transcription is silenced. The maternal mRNAs have short poly(A) tails after undergoing post-transcriptional processing necessary for stabilizing them for storage. The transcripts undergo cytoplasmic polyadenylation when they are to be translated. Transcriptome analyses comparing total mRNA and elongated poly(A) mRNA content among eggs of different quality can provide insight into molecular mechanisms affecting egg developmental competence in rainbow trout. The present study used RNA-seq to compare transcriptomes of unfertilized eggs of rainbow trout females yielding different eyeing rates, following rRNA removal and poly(A) retention for construction of the libraries. RESULTS:The percentage of embryos to reach the 32-cell stage at 24?h post fertilization was significantly correlated to family eyeing rate, indicating that inviable embryos were developmentally compromised before zygotic genome activation. RNA sequencing identified 2 differentially expressed transcripts (DETs) from total mRNA sequencing comparing females with low-quality (<?5% eyeing), medium-quality (30-50% eyeing), and high-quality (>?80% eyeing) eggs. In contrast, RNA sequencing from poly(A) captured transcripts identified 945 DETs between low- and high-quality eggs, 1012 between low- and medium-quality eggs, and only 2 between medium- and high-quality eggs. The transcripts of mitochondrial genes were enriched with polyadenylated transcript sequencing and they were significantly reduced in low-quality eggs. Similarly, mitochondrial DNA was reduced in low-quality eggs compared with medium- and high-quality eggs. The functional gene analysis classified the 945 DETs between low- and high-quality eggs into 31 functional modules, many of which were related to ribosomal and mitochondrial functions. Other modules involved transcription, translation, cell division, apoptosis, and immune responses. CONCLUSIONS:Our results indicate that differences in egg quality may be derived from differences in maternal nuclear transcript activation and cytoplasmic polyadenylation before ovulation, as opposed to accumulation and storage of maternal nuclear transcripts during oogenesis. Transcriptome comparisons suggest low-quality eggs suffered from impaired oxidative phosphorylation and translation. The DETs identified in this study provide insight into developmental competence in rainbow trout eggs.

Dataset Information

Serotypes, virulence genes and polymorphism of capsule gene cluster in Lactococcus garvieae isolated from diseased rainbow trout (Oncorhynchus mykiss) and mugger crocodile (Crocodylus palustris) in Iran.

Publications

Serotypes, virulence genes and polymorphism of capsule gene cluster in <i>Lactococcus garvieae</i> isolated from diseased rainbow trout (<i>Oncorhynchus</i> <i>mykiss</i>) and mugger crocodile (<i>Crocodylus</i> <i>palustris</i>) in Iran.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets