Dataset Information

Serotypes, virulence genes and polymorphism of capsule gene cluster in Lactococcus garvieae isolated from diseased rainbow trout (Oncorhynchus mykiss) and mugger crocodile (Crocodylus palustris) in Iran.

ABSTRACT: Background:Lactococcus garvieae causes lactococcosis in rainbow trout in many parts of the world. Aims:This study was conducted for the existence of the virulent factors and differentiation of the two serotypes in L. garvieae. Methods:Twenty-two strains of L. garvieae isolated from diseased rainbow trout from farms in different regions and mugger crocodile of Iran, were investigated. In order to rapidly detect the presence of the hly1, hly2, hly3, NADH oxidase, sod, pgm, adhPsaA, eno, LPxTG-3, adhCI, and adhCII virulence genes, two multiplex polymerase chain reaction (PCR) assays were developed. Also, simplex PCR method was used to identify the bacterial serotypes, CGC, LPxTG-2, Adhesion, and adhPav virulence genes using the specific primer. Results:All varieties of L. garvieae contained the hly1, hly2, hly3, NADH oxidase, pgm, adhPav, LPxTG-3, sod, eno, adhPsaA, adhCI, and CGC virulence genes. Also, adhCII gene was present in all strains except one of the isolates originated from mugger crocodile. In addition, LPxTG-2 gene was only present in one of the isolates belonging to mugger crocodile. Adhesion gene was not present in all the strains. Interestingly, all the 22 strains originated from both hosts were identified as belonging to the serotype I. Based on the phylogenetic sequences of the capsule gene cluster, group all fish isolates into a cluster together with one isolate obtained from mugger crocodile. Conclusion:Further studies are recommended to investigate the role of virulence genes in L. garvieae and evaluate their pathogenicity to rainbow trout.

SUBMITTER: Salighehzadeh R

PROVIDER: S-EPMC7183378 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Serotypes, virulence genes and polymorphism of capsule gene cluster in <i>Lactococcus garvieae</i> isolated from diseased rainbow trout (<i>Oncorhynchus</i> <i>mykiss</i>) and mugger crocodile (<i>Crocodylus</i> <i>palustris</i>) in Iran.

Salighehzadeh R R Sharifiyazdi H H Akhlaghi M M Soltanian S S

Iranian journal of veterinary research 20200101 1

<h4>Background</h4><i>Lactococcus garvieae</i> causes lactococcosis in rainbow trout in many parts of the world.<h4>Aims</h4>This study was conducted for the existence of the virulent factors and differentiation of the two serotypes in <i>L. garvieae</i>.<h4>Methods</h4>Twenty-two strains of <i>L. garvieae</i> isolated from diseased rainbow trout from farms in different regions and mugger crocodile of Iran, were investigated. In order to rapidly detect the presence of the <i>hly1</i>, <i>hly2</i ...[more]

PMID: 32368222

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Project description:BackgroundGenetic maps characterizing the inheritance patterns of traits and markers have been developed for a wide range of species and used to study questions in biomedicine, agriculture, ecology and evolutionary biology. The status of rainbow trout genetic maps has progressed significantly over the last decade due to interest in this species in aquaculture and sport fisheries, and as a model research organism for studies related to carcinogenesis, toxicology, comparative immunology, disease ecology, physiology and nutrition. We constructed a second generation genetic map for rainbow trout using microsatellite markers to facilitate the identification of quantitative trait loci for traits affecting aquaculture production efficiency and the extraction of comparative information from the genome sequences of model fish species.ResultsA genetic map ordering 1124 microsatellite loci spanning a sex-averaged distance of 2927.10 cM (Kosambi) and having 2.6 cM resolution was constructed by genotyping 10 parents and 150 offspring from the National Center for Cool and Cold Water Aquaculture (NCCCWA) reference family mapping panel. Microsatellite markers, representing pairs of loci resulting from an evolutionarily recent whole genome duplication event, identified 180 duplicated regions within the rainbow trout genome. Microsatellites associated with genes through expressed sequence tags or bacterial artificial chromosomes produced comparative assignments with tetraodon, zebrafish, fugu, and medaka resulting in assignments of homology for 199 loci.ConclusionThe second generation NCCCWA genetic map provides an increased microsatellite marker density and quantifies differences in recombination rate between the sexes in outbred populations. It has the potential to integrate with cytogenetic and other physical maps, identifying paralogous regions of the rainbow trout genome arising from the evolutionarily recent genome duplication event, and anchoring a comparative map with the zebrafish, medaka, tetraodon, and fugu genomes. This resource will facilitate the identification of genes affecting traits of interest through fine mapping and positional cloning of candidate genes.

Dataset Information

Serotypes, virulence genes and polymorphism of capsule gene cluster in Lactococcus garvieae isolated from diseased rainbow trout (Oncorhynchus mykiss) and mugger crocodile (Crocodylus palustris) in Iran.

Publications

Serotypes, virulence genes and polymorphism of capsule gene cluster in <i>Lactococcus garvieae</i> isolated from diseased rainbow trout (<i>Oncorhynchus</i> <i>mykiss</i>) and mugger crocodile (<i>Crocodylus</i> <i>palustris</i>) in Iran.

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