Matrix metalloproteinase-13, NF-?B p65 and interleukin-1? are associated with the severity of knee osteoarthritis.
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ABSTRACT: Knee osteoarthritis (KOA) is a prevalent disease, especially in the elderly. The present study examined the expression of matrix metalloproteinase-13 (MMP-13), NF-?Bp65 and interleukin (IL)-l? in the synovial tissues of KOA patients and the role of MMP-13 and the NF-?Bp65 signalling pathway in KOA pathogenesis. A total of 100 KOA patients were enrolled in our hospital from December 2015 to December 2017 and were classified into either a mild KOA group (Outerbridge grade 1 and 2) or a severe KOA group (Outerbridge grade 3 and 4). Non-OA patients were included as controls. Synovial tissues from patients in both groups were collected for detection of the mRNA and protein expression of MMP-13, NF-?Bp65 and IL-l?. Synovial tissue slices were subjected to haematoxylin and eosin staining and immunohistochemistry (SP method). Cartilage tissues were observed under a light microscope after Safranin O-fast green staining. Reverse transcription-quantitative PCR and western blot analyses demonstrated that the expression of MMP-13, NF-?Bp65 and IL-l? in the mild and severe groups were substantially upregulated compared with the control group (all P<0.05). A positive correlation between MMP-13 and NF-?Bp65 expression in the KOA synovial tissues was identified (P<0.05). Immunohistochemistry revealed that the expression of MMP-13 and NF-?Bp65 was related to the severity of KOA (MMP-13: severe, 92.54%; moderate, 76.52%; control: 32.14%; and NF-?Bp65: severe, 85.56%; moderate, 48.12%; control: 28.32%). This evidence indicated that the severity of KOA was related to MMP-13 and NF-?Bp65 expression. The NF-?B signalling pathway may be activated during OA progression, which could upregulate the expression of MMP-13 and IL-1? and accelerate the deterioration of articular cartilage.
SUBMITTER: Zhao QH
PROVIDER: S-EPMC7185070 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
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