Data describing the cofactor additives effect on bioelectrocatalytic activity of «crude» extracts.

ABSTRACT: «Crude» extracts obtained via simple ultrasonic disintegration of microbial cell membrane are perspective bioelectrocatalysts. This extract contains all the necessary enzymes and cofactors required for oxidative or reductive conversion. The technology of synthesis of «crude extract» is simpler and less costly in comparison with technology of obtaining pure enzymes. Dialysis of the obtained extracts was performed with different molecular weight cut-off (3.5 kDa, 12-14 kDa, 25 kDa, 50 kDa). The obtained data show that after dialysis extracts lose their dehydrogenase and bioelectrocatalytic activity due to the loss of cofactors. However, the addition of NAD and NADP cofactors leads to a recovery of activity. The obtained data demonstrate that the concentration of the cofactor directly affects the rate of the bioelectrocatalytic reaction. Also, the obtained data indicate that the composition of the enzyme systems of the extract includes succinate dehydrogenase. Analyzing this data set can provide insight on increase of the electrocatalytic activity of a new type of bioelectrocatalyst.

SUBMITTER: Dmitrieva MV 

PROVIDER: S-EPMC7186515 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature