Project description:The oligopeptide/histidine transporter SLC15A3 (PHT2) belongs to the solute carrier protein 15 family and is mainly expressed in the membrane of lysosomes and endosomes in monocytes/macrophages. Macrophages are a key immune cell population in the development of pulmonary fibrosis. In order to examine the role of SLC15A3 in pulmonary fibrosis, we build bleomycin (BLM)-induced mouse model in wild-type (WT) mice and the Slc15a3 knockout(Slc15a3-/-) mice.
Project description:The pathogenesis of idiopathic pulmonary fibrosis is multifactorial and characterized by progressive fibrosis and excessive accumulation of extracellular matrix in the interstitium of the lung, and driven by an imbalance between anti-fibrotic and pro-fibrotic factors leading to collagen deposition. In the present study we wanted to identify proteins involved in these processes, and performed high-resolution proteomic profiling of bronchoalveolar lavage (BAL) from IPF patients and controls. The proteomic analysis of BAL demonstrated that the complement system was highly differentially regulated in IPF patients as compared with controls.