Ontology highlight
ABSTRACT:
SUBMITTER: Rickner HD
PROVIDER: S-EPMC7203994 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Rickner Hannah D HD Niu Sheng-Yong SY Cheng Christine S CS
Journal of visualized experiments : JoVE 20190322 145
ATAC-seq has become a widely used methodology in the study of epigenetics due to its rapid and simple approach to mapping genome-wide accessible chromatin. In this paper we present an improved ATAC-seq protocol that reduces contaminating mitochondrial DNA reads. While previous ATAC-seq protocols have struggled with an average of 50% contaminating mitochondrial DNA reads, the optimized lysis buffer introduced in this protocol reduces mitochondrial DNA contamination to an average of 3%. This impro ...[more]